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Culture medium for cultivating white rot fungus secretion laccase

A technology of white rot fungi and culture medium, applied in the direction of fungi, oxidoreductase, and microbial-based methods, can solve the problems of low laccase production and inability to meet the requirements of mass production of laccase

Inactive Publication Date: 2008-08-06
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a culture medium for cultivating white-rot fungi secreting laccase in order to solve the problem that the laccase yield of white-rot fungi cultured in the existing culture medium is low and cannot meet the requirements for mass production of laccase

Method used

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specific Embodiment approach 1

[0009] Specific embodiment 1: The medium for cultivating white-rot fungi secreting laccase in this embodiment is that every 1000 mL of liquid medium contains 35-45 mL of corn flour extract, 0.43-0.45 g of ammonium tartrate, and 0.19-0.21 g of KH 2 PO 4 , 0.05~0.056g of MgSO 4 ·7H 2 O, 10.0~10.2mg of CaCl 2 , 0.8-1.2 mL of inorganic solution, 0.4-0.6 mL of vitamin solution and 0.2 mol pH of 7 HAc-NaAc buffer solution.

[0010] The preparation method of the corn flour leach solution of the present embodiment is as follows: boil 10 g of corn flour with 60-100 mL of water for 30 minutes, then filter out the leach solution, and control its volume to be 35-45 mL (the volume of the leach solution is greater than 45 mL, evaporate and concentrate, and the leach solution If the volume is less than 35mL, add distilled water to make up).

specific Embodiment approach 2

[0011] Specific embodiment two: the medium for cultivating white-rot fungus of the present embodiment to secrete laccase is that the liquid culture medium of every 1000mL consists of 40mL of corn flour extract, 0.44g of ammonium tartrate, and 0.2g of KH 2 PO 4 , 0.053g of MgSO 4 ·7H 2 O, 10.082 mg of CaCl 2 , 1 mL of inorganic solution, 0.5 mL of vitamin solution, 0.2 mol pH of 7 HAc-NaAc buffer solution and the rest of water.

[0012] The preparation method of the corn flour leachate in this embodiment is as follows: boil 10 g of corn flour with 80-90 mL of water for 30 minutes, then filter out the leachate, and control its volume to 40 mL.

[0013] The culture medium of the present embodiment and the existing liquid culture medium (the existing liquid culture medium of every 1000mL contains the glucose of 20g, the KH of 3g 2 PO 4 , 1.5g of MgSO 4 ·7H 2 2 and 200mL of potato extract) are all used to cultivate Trametesmetes gibbosa (Pseudotrametes gibbosa, a kind of whi...

specific Embodiment approach 3

[0014] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: MnSO in the inorganic solution 4 The concentration of NaCl is 0.5g / L, the concentration of NaCl is 1g / L, FeSO 4 ·7H 2 The concentration of O is 100mg / L, CoSO 4 The concentration is 100mg / L, ZnSO 4 The concentration is 100mg / L, CuSO 4 ·5H 2 The concentration of O is 10mg / L, AlK(SO 4 ) 2 The concentration is 10mg / L, H 3 BO 3 The concentration is 10mg / L, NaMoO 4 The concentration is 10mg / L. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a culture medium for culturing white rot fungi secretion laccase, which relates to a culture medium which cultures fungi secretion function enzyme. The invention solves the problems that white rot fungi laccase which is cultured by the existing culture medium has low laccase yield, which can not meet the requirements of producing laccase in large amount. The culture medium for culturing white rot fungi secretion laccase of the invention comprises 35-45mL corn meal leaching solution, 0.43-0.45g ammonium tartrate, 0.19-0.21g KH2PO4, 0.05-0.056g MgSO4.7H2O, 10.0-10.2mg CaCL2, 0.8-1.2mL inorganic solution, 0.4-0.6mL vitamin solution, and 0.2mL buffer solution of HAc-NaAc whose PH is 7 in each 1000mL fluid medium. The enzyme activity of laccase which is secreted by white rot fungi which is cultured by the invention is 5 times more than the existing culture mediums.

Description

technical field [0001] The invention relates to a medium for cultivating fungi secreting functional enzymes. Background technique [0002] With the development of industry, a large number of refractory organic pollutants are produced, and these pollutants can be degraded by modern biotechnology. White-rot fungi have a powerful enzyme system, which is produced by extracellular secretion, and the degradation of pollutants is mainly completed by the enzyme system, among which laccase is one of the many enzymes of white-rot fungi. Laccases (Laccases) is a protein that binds multiple copper ions, belongs to cerulus oxidase, and exists in mushrooms, fungi and plants. Laccase can survive in the air, and the only product after the reaction is water, so laccase is an environmentally friendly enzyme. [0003] Laccase plays an important role in the degradation of lignin and its precursor analogs, and can degrade more than 250 pollutants. It is widely used in pulp biological bleaching...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/04C12R1/645
Inventor 高大文梁红陈军
Owner NORTHEAST FORESTRY UNIVERSITY
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