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Kit for detecting child lead poisioning inheritance susceptible risk

A kit and children's technology, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of decreased VDR gene transcription level, affecting VDR protein activity, etc.

Inactive Publication Date: 2008-08-13
SHANGHAI HENGJIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Variations in these sites can lead to a decrease in the transcription level of the VDR gene or affect the activity of the VDR protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Use of detection kits

[0030] Step 1: Extraction of DNA template

[0031] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0032] Step 2: Real-time quantitative PCR reaction

[0033] Use a fluorescent quantitative PCR detection kit for detecting the genetic susceptibility risk of lead poisoning in children, which contains the following primer pairs and fluorescent probe pairs:

[0034] Sense primer 1: 5'-CCCCTCTACCCACACC-3' (SEQ ID NO: 1)

[0035] Antisense primer 1: 5'-GCGTAGGCCCTCTTCC-3' (SEQ ID NO: 2)

[0036] Sense primer 2: 5'-GGGAAAACTGCCCACTC-3' (SEQ ID NO: 3)

[0037] Antisense primer 2: 5'-GAAGGGTTGGCACTGAAC-3' (SEQ ID NO: 4)

[0038] Sense primer 3: 5'-GAGCAGAGCCTGAGTATT-3' (SEQ ID NO: 5)

[0039] Antisense primer 3: 5'-TTCCTGGGGCCACAGA-3' (SEQ ID NO: 6)

[0040] Sense primer 4: 5'-TGGACAGGCGGTCC-3' (SEQ ID NO: 7)

[0041] Antisense primer 4: 5'-CTGGGGTGCAGGAC-3' (SEQ ID NO: 8)

[0042] Sense primer 5: 5'-TGGA...

Embodiment 2

[0063] Example 2. The service of testing people for genetic susceptibility risk of lead poisoning in children

[0064] Step 1: DNA Extraction

[0065] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0066] Step 2: Genotyping Assays

[0067] Using the kit provided by the present invention, the SNP site No. rs1800795 on the gene encoding interleukin 6, the SNP site No. rs2073618 on the gene of osteoprotegerin TNFRSF11B, and the SNP site No. rs1800629 on the gene of tumor necrosis factor-α Sites, rs1544410 and rs731236 sites on the vitamin D receptor gene were detected by fluorescence quantitative PCR to determine the genotypes of these five SNPs sites.

[0068] Step 3: Analysis of genetic susceptibility risk of lead poisoning in children

[0069] Through the analysis of the SNPs genotypes of the teste...

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PUM

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Abstract

The invention discloses an agent for detecting baby lead poisioning descendiblity impressionable risks. The agent box comprises specificity primer pair and specificity fluorescent detecting probe pair for detecting synchronously number rs1800435 SNP site on Delta amidocyanogen-Gamma ketone pentanoic acid dehydrogenase gene, number rs1800802 SNP site on ground mass Gamma-carboxy group aminoglutaric acid egg albumen gene, number rs1544410 and rs731236 SNP site on vitamin D receptor gene, number rs1799782 SNP site on X ray interlacing complementation repair gene 1, general component for detecting fluorescent definite quantity PCR etc.. The agent box of the invention assesses baby lead poisioning descendiblity impressionable risks by detecting synchronously mononucleotide polymorphism site gene type correlative closely to baby lead poisioning descendiblity impressionable risks.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting the genetic susceptibility risk of lead poisoning in children, through simultaneous detection of δamino-γ ketones closely related to the genetic susceptibility risk of lead poisoning in children Single nucleotide polymorphisms in valerate dehydrogenase gene (ALAD), matrix γ-carboxyglutamate protein gene (MGP), vitamin D receptor gene (VDR) and X-ray staggered complementation repair gene 1 (XRCC1) Locus genotype to assess the genetic susceptibility risk of lead poisoning in children. Background technique [0002] Lead poisoning is a type of poisoning caused by environmental pollution and bad living habits, which can easily affect the health of children and adolescents. As children are in the growth and development stage, they are prone to lead poisoning due to their unique physiological characteristics. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 冯哲民邹祖烨
Owner SHANGHAI HENGJIAN BIOTECH CO LTD
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