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Method for improving shikimic acid yield by fermentation method

A kind of technology of shikimic acid, fermentation method, be applied in the field of obtaining shikimic acid, can solve the problems such as improving shikimic acid output, reach the effect that production conditions are stable and reliable, improve output, reduce the generation of by-products

Inactive Publication Date: 2011-06-01
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No one has yet used blockers for biosynthetic processes to increase shikimic acid production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Seed culture: Brevibacterium lactose fermenterum was inoculated into the seed medium, and the components and contents of the seed medium were: glucose 28g, ammonium sulfate 20g, potassium dihydrogen phosphate 1.8g, magnesium sulfate 0.4g, urea 1.8g, Take 20g of corn steep liquor, adjust the volume to 1L with water, adjust the pH to pH7.0, sterilize at 115°C for 30min, and fill the liquid with a volume of 25mL / 250Ml. Cultivate at 30°C, 120rpm shaker for 16h.

[0024] (2) Fermentation culture:

[0025] The Brevibacterium lactose fermenterum culture solution obtained through seed cultivation is inoculated into the fermentation medium of pH7.0 according to the inoculum size of 5%, and the components and contents of the fermentation medium are: wherein the components and contents of the fermentation medium (g / L ) is: glucose 140, ammonium sulfate 45, potassium dihydrogen phosphate 1.5, magnesium sulfate 1.0, corn steep liquor 20, calcium carbonate 30, dilute to 1 L with...

Embodiment 2

[0029] (1) Seed cultivation: inoculate Brevibacterium lactose fermenterum into the seed medium, the components and content of the seed medium are: the components and content (g / L) of the seed medium are: glucose 30, ammonium sulfate 20, phosphoric acid Potassium dihydrogen 1.5, magnesium sulfate 0.5, urea 1.5, corn steep liquor 25, dilute to 1L with water, adjust the pH to pH 7.4, sterilize at 115°C for 30min, and the liquid volume is 25mL / 250Ml. Cultivate for 14 h at 30° C. in a shaker at 140 rpm.

[0030] (2) Fermentation culture:

[0031] The Brevibacterium lactose fermenterum culture solution obtained through seed cultivation is inoculated into the fermentation medium of pH7.4 according to the inoculum size of 5%, and the components and contents of the fermentation medium are: wherein the components and contents of the fermentation medium (g / L ) is: glucose 130, ammonium sulfate 40, potassium dihydrogen phosphate 1.5, magnesium sulfate 0.5, corn steep liquor 25, calcium c...

Embodiment 3

[0035] (1) Seed cultivation: inoculate Brevibacterium lactose fermenterum into the seed medium, the components and content of the seed medium are: the components and content of the seed medium are: glucose 32g, ammonium sulfate 15g, potassium dihydrogen phosphate 2.0g , magnesium sulfate 0.8g, urea 0.7g, corn steep liquor 30g, dilute to 1L with water, adjust the pH to pH7.4, sterilize at 115°C for 30min, the filling volume is 25mL / 250Ml. Incubate at 30°C for 12 hours in a shaker at 160 rpm.

[0036] (2) Fermentation culture:

[0037] Inoculate the culture solution of Brevibacterium lactose fermenterum obtained through seed cultivation into the fermentation medium of pH 7.4 according to the inoculum size of 5%. The components and content of the fermentation medium are: wherein the components and content of the fermentation medium are: glucose 160g , ammonium sulfate 32g, potassium dihydrogen phosphate 0.8g, magnesium sulfate 0.3g, corn steep liquor 30g, calcium carbonate 50g, ...

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PUM

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Abstract

A method for improving the yield of a fermentation method for producing shikimic acid pertains to the biochemical field. The invention comprises the steps that: 1) Seed culture: a lactose fermentation bacillus brevis is inoculated in a seed culture medium of pH7.0 to pH7.4, the culture is carried out in a 120 to 180rpm shake bed for 12 to 18 hours at 30 DEG C; the seed culture medium (g / L) includes: 25 to 35 parts of glucose, 15 to 25 parts of ammonium sulfate, 1.0 to 2.0 parts of potassium dihydrogen phosphate, 0.1 to 1.0 part of magnesium sulfate, 0.5 to 2.0 parts of urea, 20 to 30 parts of corn pulp and the rest of water, the pH is regulated to pH 7.0 to pH 7.4, and the sterilization is carried out for 30 minutes at 115 DEG C. 2) Fermentation culture: the seed is inoculated in a fermentation culture medium at pH7.0 to pH7.4, the inoculation amount is 5 percent, the culture is carried out in the 120 to 180rpm shake bed for 48 to 60 hours at 30 DEG C; a glyphosate inhibitor is addedtill the fermentation is going on for 16 to 30 hours; the fermentation culture medium (g / L) includes: 100 to 160 parts of glucose, 30 to 50 parts of ammonium sulfate, 0.5 to 2.0 parts of potassium dihydrogen phosphate, 0.1 to 1.0 part of magnesium sulfate, 20 to 30 parts of corn pulp, 30 to 50 parts of calcium carbonate and the rest of water, the pH is regulated to pH7.0 to pH7.4, and the sterilization is carried out for 30 minutes at 115 DEG C. The yield of the shikimic acid can achieve 1.667 to 2.887 times of the yield before the adding of the glyphosate, and the production is stable and reliable.

Description

technical field [0001] The invention relates to a new method for obtaining shikimic acid, in particular to a new method for obtaining shikimic acid by fermenting Brevibacterium lactose fermentation, belonging to the field of biochemical industry. Background technique [0002] Shikimic acid is an important intermediate in the biosynthetic pathway of aromatic amino acids and aromatic vitamins - the shikimic acid pathway. According to the pharmacological research on shikimic acid, shikimic acid inhibits platelet aggregation by affecting the metabolism of arachidonic acid, thereby inhibiting arterial and venous thrombosis and cerebral thrombosis; it has anti-inflammatory and analgesic effects. Shikimic acid can also be used as an intermediate of antiviral and anticancer drugs. It is a raw material for the synthesis of antitumor drugs such as dioxin and glyoxalase inhibitors. It is also a synthetic neuraminidase inhibitor oseltamivir phosphate ( oseltamivir phosphate, trade name...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/42C12R1/13
Inventor 袁其朋邓美飞程艳
Owner BEIJING UNIV OF CHEM TECH