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A flow analyzer and biological technology, applied in the field of analyzers, can solve the problems of large amount of samples and reagents, low detection sensitivity, bulky instrument volume, etc., and achieve the effect of compact structure, less consumption, and small volume
Inactive Publication Date: 2008-08-27
XIAMEN UNIV
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[0007] The purpose of the present invention is to provide a biological flow cytometer with ultra-high sensitivity for the shortcomings of the existing conventional flow cytometer, such as low detection sensitivity, bulky instrument, complicated operation, high price, and large amount of samples and reagents. Analyzer
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[0016] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.
[0017] Such as figure 1 As shown, the present invention includes an optical system A, a liquid flow system B and a detection system C.
[0018]Optical system A is equipped with an optical platform, a solid-state laser 2, a translation stage, a continuously adjustable attenuator 4, a mirror 5, an achromatic doublet lens 6, a quartz flow cell 7, a first aspheric lens 8, and a second aspheric lens 9 , the third aspheric lens 10, the first filter 11, the second filter 12 and the third filter 13. The solid-state laser 2, the achromatic doublet lens 6, and the quartz flow cell 7 are respectively fixed on the optical platform through a translation stage, the continuously adjustable attenuator 4, and the reflector 5 are fixed on the optical platform through a pole, and the first aspheric lens 8, The second aspheric lens 9, the third aspheric lens 10...
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Abstract
A biological flow type analyzer relates to an analyzer, in particular to a biological flow type analyzer and application thereof. The present invention provides a biological flow type analyzer high in sensitivity, comprising an optical system, a liquid flow system and a detection system, wherein the optical system is provided with an optical platform, a solid-state laser, a translation table, a continuous-adjustable attenuator, a reflecting mirror, an achromatic scuffing lens, a quartz circulating pool, a first aspheric lens, a second aspheric lens, a third aspheric lens, a first optical filter, a second optical filter and a third optical filter; the liquid flow system is provided with a nitrogen cylinder, a triple valve, a first switch valve, a second switch valve, a third switch valve, a fourth switch valve, a pressure regulator, a pressure sensor, a four-way valve, an injector, a quartz capillary tube, a sample tube, a sample injector, a sheath liquid bottle and a waste liquid bottle; the detection system is provided with a first detector, a second detector, a translation table, a data acquisition card and signal acquisition software.
Description
technical field [0001] The invention relates to an analyzer, in particular to a biological flow analyzer and its application. Background technique [0002] Flow cytometry is an instrument for quantitative analysis and sorting of single cells or other biological particles at the functional level. The test sample is pressed into the flow chamber, and the cell-free phosphate buffer is sprayed out from the sheath fluid tube under high pressure to form a flow stream of a certain shape. The cells to be tested are arranged in a single row under the coating of the sheath fluid and pass through the laser detection area in turn. . The fluorescently stained cells produce scattered light and fluorescence under the irradiation of the laser beam, and the light signals are received by the photomultiplier tubes in the forward and 90° directions at the same time. The liquid phase chip analysis system is a new generation of molecular diagnostic technology platform that combines flow cytomet...
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