Sudan red 1 immunity-chromatography test paper detection method

Abstract

The invention discloses a Sudan I immune chromatography test paper detection method. On the back liner of the test paper, a sample liquor absorption part, a colloidal gold marking part, a detecting reaction part and a water absorption part are sequentially pasted, wherein, the detecting reaction part is covered with one or two strips of Sudan I polyclonal antibodies or one or two strips of antigens used for detecting, which are used as detecting lines, meanwhile, one strip of IgG which resists the second species animal protein also covers the detecting reaction part and is used as a reference line. The combination mode has two ways, that is, one detecting line plus one reference line or two detecting lines plus one reference line. The fast detection test paper can be used when the temperature ranges from 4 DEG C to 40 DEG C and is suitable for fast detecting foods such as meat, etc. which may be polluted by Sudan I by departments such as sanitary, quality supervision, customs and supermarkets, etc. The Sudan I immune chromatography test paper detection method has the advantages of strong specificity, high sensitivity, capability for realizing semi-quantitative detection, simple and convenient operation, etc.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for detecting harmful residues in food, in particular to a method for detecting Sudan red I test paper. Background technique [0002] Sudan red is a lipophilic azo compound, mainly including four types: I, II, III and IV. Because of its bright red color, Sudan Red is often added to food by unscrupulous merchants to increase the freshness of food appearance and attract consumers, among which Sudan I (Sudan I) is the most common. The chemical name of Sudan Red I is 1-phenylazo-2-naphthalenol (1-phenylazo-2-naphthalenol), and its molecular structure is C 6 h 5 =NC 10 h 6 OH, molecular weight 248.28. Aniline, a moderately toxic carcinogen produced by the degradation of Sudan Red I during its metabolism, can cause toxic liver disease, and long-term intake of aniline can also cause damage to the human nervous system. my country's "Hygienic Standards for the Use of...

AI Technical Summary

Problems solved by technology

However, when using HPLC or LC-MS, the sample pretreatment is cumbersome, the determination method is complicated, and it needs specially trained personnel to operate, and there are problems such as narrow application range, expensive equipment, and high cost.

At present, there is no detection method with strong specificity, high sensitivity, semi-quantitative detection, and simple and convenient operation in this technical field.

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