Preparation of loquat leaf total flavones and functions thereof for reducing blood sugar and blood fat

A technology of total flavonoids and loquat leaves, which is applied in the field of blood lipid metabolism disorders, and can solve the problems that there is no preparation process of total flavonoids from loquat leaves, and the total flavonoids can lower blood sugar, blood lipids, blood lipid metabolism disorders, etc.

Inactive Publication Date: 2008-11-12
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is reported that loquat leaves contain a variety of flavonoids, but there are no reports on the preparation process of total flavonoids from loquat leaves, the hypoglycemic and lipid-lowering functions of total flavonoids, and the use in treating diabetes and improving blood lipid metabolism disorders caused by diabetes

Method used

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  • Preparation of loquat leaf total flavones and functions thereof for reducing blood sugar and blood fat
  • Preparation of loquat leaf total flavones and functions thereof for reducing blood sugar and blood fat
  • Preparation of loquat leaf total flavones and functions thereof for reducing blood sugar and blood fat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: 1.0 kg of dried loquat leaves were pulverized into coarse powder, cold soaked twice with 80% ethanol for 15 days each time, combined soaking liquid, concentrated under reduced pressure to become a thick paste, stirred with water, and cooled. Place in a centrifuge to settle and centrifuge, and take the supernatant for later use.

[0046]Take the macroporous adsorption resin, pack it into a column at a ratio of 1:2 to the weight ratio of the crude drug, load the above-mentioned spare supernatant, first elute with deionized water, and then use 20%-60% ethanol for gradient elution, The ethanol eluate was collected, concentrated under reduced pressure, and dried to obtain the total flavonoids extract of loquat leaves with a yield of 1.4%.

Embodiment 2

[0047] Example 2: 1.0 kg of dried loquat leaves were pulverized into coarse powder, heated and refluxed with 80% ethanol for extraction twice, combined extracts, concentrated under reduced pressure to form a thick paste, stirred with water, and cooled. Place in a centrifuge to settle and centrifuge, and take the supernatant for later use.

[0048] Take the macroporous adsorption resin, pack it into a column at a ratio of 1:1 to the weight ratio of the crude drug, load the above-mentioned standby supernatant, first elute with deionized water, and then use 20%-70% alcohol for gradient elution, The ethanol eluate was collected, concentrated under reduced pressure, and dried to obtain the total flavonoids extract of loquat leaves with a yield of 1.5%.

Embodiment 3

[0049] Example 3: 1.0 kg of dried loquat leaves were ground into coarse powder, impregnated with 60% ethanol for 48 hours, extracted by percolation, until the color of the effluent liquid was light, and the reaction of flavonoids was not obvious, concentrated under reduced pressure to form a thick paste, and stirred , calm. Place in a centrifuge to settle and centrifuge, and take the supernatant for later use.

[0050] Take the macroporous adsorption resin, pack it into a column at a ratio of 1:1 to the weight ratio of the crude drug, load the above-mentioned spare supernatant, first elute with deionized water, and then use 30%-80% ethanol for gradient elution, The ethanol eluate was collected, concentrated under reduced pressure, and dried to obtain the total flavonoids extract of loquat leaves with a yield of 1.6%.

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PUM

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Abstract

The invention relates to a method for preparing folium eriobotryae total flavonoids and hypoglycemic and antilipemic functions, and provides a method for extracting and preparing the total flavonoids from folium eriobotryae and functions and use of the total flavonoids prepared by the method. Firstly, the method for preparing the folium eriobotryae total flavonoids is as follows: folium eriobotryae medicinal materials undergo reflux extraction, soaking extraction or percolation extraction through alcohols or methanol; extracting solution is decompressed, concentrated, precipitated after addition of water and then centrifuged; macroporous resins or polyamide columns are added into supernatant; after elution of impurities by water, gradient elution of the supernatant is performed by alcohols; alcohol eluent is collected, decompressed, concentrated, dried and crushed, and then folium eriobotryae total flavonoid extract is obtained. Secondly, the quality standard of the folium eriobotryae total flavonoids is as follows: the ultraviolet spectrophotometric method is used for determination; NaNO2-Al (NO3) 3-NaOH is used for color development; and rutoside is used for calculation, the total content of the flavonoids is not lower than 50 percent. Thirdly, the functions of the folium eriobotryae total flavonoids are as follows: the functions are hypoglycemic and antilipemic functions, and the use is to cure diabetes and blood-fat metabolic disturbance caused by the diabetes.

Description

Technical field: [0001] The invention relates to a method for extracting and preparing total flavonoids from traditional Chinese medicine loquat leaves, the function of lowering blood sugar and blood fat of the extract, and the application in treating diabetes and improving blood lipid metabolism disorder caused by diabetes. Background technique: [0002] Diabetes is a common endocrine and metabolic disease, which belongs to the category of diabetes in traditional Chinese medicine, and is the third major disease that endangers human health after cardiovascular disease and cancer. According to the World Health Organization report, there are as many as 125 million people with diabetes worldwide, and this number is expected to increase to 299 million by 2025. Due to the large population base, there are more than 30 million diabetic patients in my country, and the absolute number of patients ranks first in the world. Because diabetes has the characteristics of high prevalence r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P3/06A61P3/10A61K127/00
Inventor 李维林吕寒陈剑吴菊兰任冰如
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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