Method for preventing and controlling stripe leaf blight of rice with biological vaccine seed presoaking technique

A technology for rice stripe and leaf blight, which is applied in the field of rice disease control, can solve problems such as the inoperability of rice stripe blight, and achieves the effect of strong permeability

Inactive Publication Date: 2008-12-10
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the biological vaccines developed by gene interference technology (RNAi) can only enter the target organisms through injection, feeding, gene gun, transgenic,

Method used

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  • Method for preventing and controlling stripe leaf blight of rice with biological vaccine seed presoaking technique
  • Method for preventing and controlling stripe leaf blight of rice with biological vaccine seed presoaking technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] When soaking rice seeds, different doses of osmotic agents were added instead of water by biological vaccines, and the effects of different concentrations of several osmotic agents on rice germination and emergence were studied (Yangzhou, Jiangsu). The results are shown in Tables 1 and 2.

[0022] Table 1 Test of germination rate and emergence rate of 200 grains of paddy (rice seeds were rinsed first to remove shrunken grains, 3.10-18)

[0023]

[0024] Note: 1) The germination rate is measured after 48 hours of soaking and 24 hours of accelerated germination, and the emergence rate is the result of inspection 4-5 days after sowing.

[0025] Table 2 Treatment results of high-concentration osmotic agent (100 grains, 3.10-18) (200 grains for two controls, 100 grains for the others)

[0026]

[0027] The test results show that the concentration of penetrant added to the biological vaccine is between 0.01%-0.1% (volume), which has little effect on the germination and ...

Embodiment 2

[0028] Example 2: Determination of residues of "biological vaccine" in rice plants after rice soaking and emergence (Yangzhou, Jiangsu)

[0029] The rice seedlings soaked in biological vaccines were planted in barrels, and PCR technology was used to detect whether the rice plants still contained "biological vaccines" 15 days and 35 days after emergence. the result shows:

[0030] After 15 days: control vaccine (0 / 9), control water (0 / 9), C1 (6 / 9), C0.5 (0 / 9), C0.1 (0 / 9), B1 (1 / 9) , B0.5(0 / 9), B0.1(6 / 9), A1(7 / 9), A0.5(3 / 9), A0.1(1 / 9) (Note: 1 / 9 9 rice seedlings were tested, and 1 plant contained the vaccine)

[0031] After 35 days: C1(7 / 10), C0.5(3 / 10), C0.1(7 / 10), B1(2 / 10), B0.5(5 / 10), B0.1(7 / 10 10), A1(6 / 10), A0.5(4 / 10), A0.1(7 / 10)

[0032] (Note: 1 / 10 means that 10 rice seedlings were tested, and 1 plant contained the vaccine, C1 means that 1 / 1000 (volume) of C penetrant was added, and so on)

Embodiment 3

[0033] Embodiment 3: the effect measurement of biological vaccine seed soaking technology preventing and treating stripe leaf blight (Yangzhou, Jiangsu)

[0034] The cement pond was used as the experimental site, rice with different treatments was sown, and the disease incidence of rice plants was systematically checked after emergence. The following are the inspection results at 83 days after emergence:

[0035] deal with

Total number of holes

Achilles

Corrected dead seedling rate% (clear water)

Corrected dead vaccine % (vaccine)

A0.5

25

0

0

0

A2

16

0

0

0

B1

20

0

0

0

B4

25

0

0

0

C4

20

1

16.7

45.0

C2

15

1

22.2

60.1

C0.1

19

2

35.1

94.8

A4

20

1

16.7

45.0

vaccine

27

3

-

-

deal with

Total number of hol...

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PUM

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Abstract

The invention relates to the field of rice disease control, in particular to a control method for the rice stripe disease. The control method is characterized in that water is replaced by biological vaccines as well as penetrating agent when the rice seeds are being dipped; the biological vaccines infiltrates through the rice in a seed dipping type; furthermore, the biological vaccines are expressed inside the rice bodies. The strip disease cavity (branch) rate of the rice which is disposed by the seed dipping of the biological vaccines is reduced by more than 65% compared with the contrast, and is reduced by more than 40% compared with the that of the rice without penetrating agent, which presents better effect for the strip disease control.

Description

technical field [0001] The invention relates to the field of rice disease prevention and control, in particular to a method for preventing and treating rice stripe leaf blight. Background technique [0002] Rice stripe blight is a viral disease caused by stripe virus (RSV) transmitted by pests such as SBPH. Since the end of the last century, the viral disease has re-emerged in Jiangsu and surrounding areas, and has become the most important disease in rice production in Jiangsu in recent years, causing disastrous effects on rice production and farmers' economic interests in Jiangsu Province. In recent years, the stripe leaf blight caused by SBPH has caused an annual loss of nearly 10 billion yuan to the economy of Jiangsu. Due to the diversification of rice varieties and cultivation systems, as well as the continuous accumulation of stripe virus sources, the occurrence of rice stripe blight will continue to be serious. In terms of production, in order to prevent and contro...

Claims

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Application Information

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IPC IPC(8): A01C1/08
Inventor 杨益众梁国华周勇王爽
Owner YANGZHOU UNIV
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