Method for rapidly manufacturing large tissue specimen slice

A tissue specimen and production method technology, applied in the field of specimen processing, can solve the problems of long production process cycle of large slices, difficult to make high-quality slices, difficult to process large slices, etc., so as to shorten the processing time, reduce errors, and reduce differences sexual effect

Inactive Publication Date: 2008-12-17
孙翔宇
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Problems solved by technology

However, the production process of large slices is long and difficult, and it is difficult to make high-quality slices. To make large slices, large tissue specimens must be processed first. However, the traditional large tissue specimen processing method has a long cycle, poor production effect, and is not easy to process. Produce large slices that meet the requirements

Method used

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with embodiment.

[0030] 1) Use 10% formaldehyde solution to fix the specimen with a thickness less than 4mm; the fixation time is according to the formula t=V 大 / V s ·Z·F g / 10, where t is the tissue fixation time, V 大 is the volume of large tissue, V s It is the volume of conventional sampling, which is a constant of 0.1×0.2×0.1, Z is 1.1 is the number of times that formaldehyde penetrates the first 1mm of tissue thickness, F is the thickness of tissue sampling 3-5mm, and g is 1.2 is the formaldehyde in the tissue. the diffusion speed;

[0031] 2) Dehydration, choose gradient ethanol, its concentration starts from 70% to the position of absolute ethanol, dehydration time according to the formula t 脱水时间 =V 脱大 / V 脱s ·Z 脱 ·F 脱 g 脱 , where Z 脱 It is the time shown for the first 1mm of ethanol penetrating tissue, and its value is 2; F 脱 The tissue thickness is 3-5mm, g 脱 is the ethanol dif...

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Abstract

The invention discloses a method for rapidly preparing a large tissue specimen slice. The method is simple, and the treatment process is rapid; furthermore, the method can meet the requirement of preparing the large tissue slice. The process comprises the following steps: (1) fixation: a specimen with the thickness smaller than 4mm is fixed by adopting 10 percent of formalin; (2) dehydration: gradient ethanol is selected for dehydration; (3) transparentization: chloroform or dimethyl benzene is adopted to perform transparentizing treatment; (4) wax impregnation; (5) embedment; (6) slicing; (7) coloration: the rapid preparation of the large tissue specimen slice is completed.

Description

technical field [0001] The invention relates to a specimen processing method, in particular to a rapid preparation method of large tissue specimen slices. Background technique [0002] In the medical field, it is often necessary to make paraffin sections in order to observe the changes of cells and tissue physiology and pathology, and the tissue samples need to be processed before making the paraffin sections. Since the usual paraffin sections are relatively small, generally within 2.5cm×1.5cm×0.1cm, there are certain limitations in observing the tissue structure under a microscope. If a large tissue section is used, it is easier to observe and make an accurate judgment. However, the production process of large slices is long and difficult, and it is not easy to make high-quality slices. To make large slices, large tissue samples must be processed first. However, the traditional large tissue sample processing method has a long cycle, poor production effect, and is not easy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 孙翔宇张朝蓬孙晓荣李新科孙兰萍
Owner 孙翔宇
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