Method for rapidly manufacturing large tissue specimen slice
A tissue specimen and production method technology, applied in the field of specimen processing, can solve the problems of long production process cycle of large slices, difficult to make high-quality slices, difficult to process large slices, etc., so as to shorten the processing time, reduce errors, and reduce differences sexual effect
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[0029] The present invention will be further described below in conjunction with embodiment.
[0030] 1) Use 10% formaldehyde solution to fix the specimen with a thickness less than 4mm; the fixation time is according to the formula t=V 大 / V s ·Z·F g / 10, where t is the tissue fixation time, V 大 is the volume of large tissue, V s It is the volume of conventional sampling, which is a constant of 0.1×0.2×0.1, Z is 1.1 is the number of times that formaldehyde penetrates the first 1mm of tissue thickness, F is the thickness of tissue sampling 3-5mm, and g is 1.2 is the formaldehyde in the tissue. the diffusion speed;
[0031] 2) Dehydration, choose gradient ethanol, its concentration starts from 70% to the position of absolute ethanol, dehydration time according to the formula t 脱水时间 =V 脱大 / V 脱s ·Z 脱 ·F 脱 g 脱 , where Z 脱 It is the time shown for the first 1mm of ethanol penetrating tissue, and its value is 2; F 脱 The tissue thickness is 3-5mm, g 脱 is the ethanol dif...
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