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Use of lipoamide for preventing and treating age related macular degeneration

A technology for lipoamide, macular degeneration, applied in the field of pharmacy

Inactive Publication Date: 2011-05-18
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some studies have shown that acrolein is more toxic (about 10-1000 times) than toxins such as formaldehyde and acetaldehyde, and is more likely to cause cell damage

Method used

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  • Use of lipoamide for preventing and treating age related macular degeneration
  • Use of lipoamide for preventing and treating age related macular degeneration
  • Use of lipoamide for preventing and treating age related macular degeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0144] ARPE-19 cells were treated with 40 μM lipoamide (LM), 75 μM acrolein (A) and 40 μM lipoamide+75 μM acrolein (LM+A), respectively, and detected by tetramethylazoblue (MTT) method Cell activity, the cells without lipoamide and acrolein (C) were used as the control. The results are shown in Figure 1.

[0145] like Figure 1A It shows that after 24 hours of single addition of acrolein (Acrolein, A), the cell viability drops to 13% of the control (Control, C), while the cell viability of the cells treated with acrolein (LM+A) protected by adding lipoamide in advance is significantly higher In the acrolein treatment group, there was no significant change compared with the control group. **p<0.01, compared with the control group; ##p<0.01, compared with the acrolein group, the experimental results of more than three times were used for statistical testing.

[0146] Photographs taken with an inverted fluorescence microscope Figure 1B As shown, in terms of cell morphology, ...

Embodiment 2

[0149] After ARPE-19 cells were treated with 40 μM lipoamide (LM), 75 μM acrolein (A) and 40 μM lipoamide + 75 μM acrolein (LM+A) for 24 hours, the mitochondrial membrane potential was detected, and without lipoic acid Cells containing amide and acrolein (C) served as controls. The results are shown in Figure 2.

[0150] Depend on Figure 2A As shown, after adding acrolein (A) for 24 hours, the membrane potential of mitochondria decreased to 37% of that of the control group (C), while the mitochondria of cells treated with acrolein (LM+A) The membrane potential was significantly higher than that of the acrolein-treated group, but there was no significant change compared with the control group. **p<0.01, compared with the control group; ##p<0.01, compared with the acrolein group, the experimental results of more than three times were used for statistical testing.

[0151] Photographs taken with a fluorescence microscope Figure 2B , it can be seen that after adding acrolein...

Embodiment 3

[0154] After ARPE-19 cells were treated with 40 μM lipoamide (LM), 75 μM acrolein (A) and 40 μM lipoamide+75 μM acrolein (LM+A) for 24 hours, the activities of mitochondrial complexes I and II were detected, The cells (C) without lipoamide and acrolein were used as the control. The results are shown in Figure 3.

[0155] Depend on Figure 3A and Figure 3B It can be seen that after adding acrolein (A) for 24 hours, the activities of complexes I and II of the mitochondria were reduced to 34% and 38% of the control group (C), respectively, while the cells treated with acrolein pre-protected with lipoamide The activities of mitochondrial complexes I and II in (LM+A) were significantly higher than those in the acrolein-treated group, even exceeding the level of the control group. **p<0.01, *p<0.05, compared with the control group; ##p<0.01, #p<0.05, compared with the acrolein group, more than three experimental results were used for statistical testing.

[0156] From the above...

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Abstract

The invention belongs to the field of pharmacy, disclosing a novel purpose of lipoamide which is used for preparing a preparation for preventing or curing diseases being relevant to cell trauma. The invention discloses the novel purpose at the aspect of preventing and curing cell trauma for the first time; in addition, the extremely effective easing and curing function of the lipoamide to the trauma of retinal pigment epithelium cell is disclosed for the first time, the pharmacological actions are very obvious, no any toxic and side effects appear and the admirable medicated prospect can be reached.

Description

technical field [0001] The invention belongs to the field of pharmacy, and more specifically, the invention relates to the application of lipoamide in preventing and treating cell damage. Background technique [0002] Cells are the basic unit of the body, and damage to cells can lead to various injuries or diseases in the body. Many external factors (such as formaldehyde, acrolein) or internal factors can cause cell damage in the body. Cell damage is often manifested as DNA damage, protein oxidation, mitochondrial function decline, intracellular redox balance disruption, etc., resulting in a variety of diseases occurrence, such as macular disease. It has been reported that acrolein can cause mitochondrial dysfunction in many tissues and cells, such as liver, lung, brain and Chinese hamster cells. [0003] Evidence has shown that mitochondria accumulate oxidative damage as the body ages, and mitochondrial dysfunction contributes to aging and degenerative diseases. Many vi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/381A61P27/02A61P43/00
Inventor 刘健康李雪森刘中博
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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