Selaginella uncinata extraction, its preparation method and application
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A technology of green cloud grass and extract, applied in the field of traditional Chinese medicine, can solve problems such as virus infection
Inactive Publication Date: 2009-02-04
JINAN UNIVERSITY
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[0016] 12. Virus infection
Method used
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Embodiment 1
[0037] (1) Grinding 4.2 kg of the dried whole herb of Cuiyuncao, extracting 2 times with 60% ethanol Soxhlet with 8 times the weight of Cuiyuncao, each time for 1 hour, the extraction temperature is 55°C, and the extracts are combined;
[0038] (2) remove insoluble matter by filtration, dry under reduced pressure, obtain 856g of the first extract of Cuiyuncao;
[0039] (3) Get 800g of the first extract of Cuiyuncao and carry out macroporous resin open column chromatography, elute with 30% ethanol aqueous solution to obtain 500g of Cuiyuncao non-flavonoid extract, use 95% ethanol The aqueous solution was eluted to obtain 250 g of Cuiyuncao total flavonoids extract.
Embodiment 2
[0041] (1) Grinding 2.0 kg of the dried whole herb of Cuiyuncao, leaching and extracting with 20% methanol 9 times the weight of Cuiyuncao 3 times, each time for 4 hours, the extraction temperature is 25°C, and the extracts are combined;
[0042] (2) remove insoluble matter by filtration, dry under reduced pressure, obtain 382g of the first extract of Cuiyuncao;
[0043] (3) Get 330g of the first extract of Cuiyuncao and carry out macroporous resin open column chromatography, elute with 30% ethanol aqueous solution to obtain 210g of Cuiyuncao non-flavonoid extract, use 95% ethanol The aqueous solution was eluted to obtain 93 g of the total flavonoids extract of Cuiyuncao.
Embodiment 3
[0045] (1) Grinding 2.0 kg of the dried whole herb of Cuiyuncao, extracting 6 times with hydrothermal reflux of 10 times the weight of Cuiyuncao, 5 hours each time, the extraction temperature is 95°C, and the extracts are combined;
[0046] (2) remove insolubles by filtration, and dry under reduced pressure to obtain 446 g of the initial extract of Cuiyuncao;
[0047] (3) Get 400g of the first extract of Cuiyuncao and carry out macroporous resin open column chromatography, elute with 30% ethanol aqueous solution to obtain 262g of Cuiyuncao non-flavonoid extract, use 95% ethanol The aqueous solution was eluted to obtain 105 g of the total flavonoids extract of Cuiyuncao.
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Abstract
The invention provides an application of an uncinata spikemoss herb extract in preparing an anti-hypoxic and an anti-oxidation medicine. The uncinata spikemoss herb extract contains a non-flavonoid extract of the uncinata spikemoss herb or a total flavonoid extract of the uncinata spikemoss herb or the mixture of the two. The invention further provides a preparation method of the uncinata spikemoss herb extract, which comprises the following steps: the dry uncinata spikemoss herb is crushed and extracted by refluxing with an aqueous solvent to obtain an extracting solution; the extracting solution is filtrated or centrifuged to remove insoluble substances, and then decompressed and dried to obtain the crude extract of the uncinata spikemoss herb; then macroporous resin open column chromatography is applied to the crude extract of the uncinata spikemoss herb, and the crude extract is eluted with ethanol aqueous solution with the volume percent being 30 percent so as to obtain the non-flavonoid extract of the uncinata spikemoss herb, and eluted with the ethanol aqueous solution with the volume percent being 30 percent to obtain the total flavonoid extract of the uncinata spikemoss herb.
Description
technical field [0001] The invention provides an extract of Cuiyuncao and its preparation method and application, belonging to the field of traditional Chinese medicine. Background technique [0002] Hypoxia refers to oxygen deficiency, which is the general term for the state of lack of oxygen or oxygen in the air. When the body is hypoxic, it will cause changes in functional metabolism, including the body's compensatory response to hypoxia and the metabolism and dysfunction caused by hypoxia. It will have varying degrees of impact on the body's respiratory system, circulatory system, blood system and other systems. Half of my country's land area is located in the west, where the natural environment is relatively harsh. Natural factors such as plateau hypoxia have seriously affected the living, survival, development and development of the people here. In particular, preliminary surveys in the "roof of the world" Qinghai-Tibet Plateau and Pamirs, which account for nearly 1 / ...
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