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Bacillus subtilis, seed-dressing agent and use thereof

A Bacillus subtilis, spore count technology, applied in the field of biological pesticide products, can solve the problems of slow development, environmental pollution, personal safety, etc., and achieve the effect of strong antibacterial activity

Inactive Publication Date: 2011-08-24
武汉天惠生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This will not only bring personal safety problems to production and users, but also cause pollution to the environment.
Biological high-efficiency and low-toxicity seed coating agents have become the focus of many people's attention, but the development is slow

Method used

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  • Bacillus subtilis, seed-dressing agent and use thereof
  • Bacillus subtilis, seed-dressing agent and use thereof
  • Bacillus subtilis, seed-dressing agent and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The formula of the seed coating agent of the present embodiment is: BSY2 mother powder 20.22%, thiophanate-methyl 0.93%, α-naphthalene acetic acid 0.004%, gibberellin 0.0091%, diatomaceous earth 60.65%, sodium carboxymethyl cellulose 16.17% %, sodium lignosulfonate 2.02%.

[0041] Accurately weigh 20.22 kilograms of BSY2 mother powder (the mother powder is prepared by Experimental Example 2, the number of viable spores is 90 billion cfu / gram, and the fineness is 160 mesh), thiophanate-methyl (Nippon Soda Co., Ltd., 90% of the original drug, 100% Total) 0.93 kg, sodium lignosulfonate 2.02 kg, sodium carboxymethyl cellulose 16.17 kg, diatomaceous earth 60.65 kg, gibberellin 9.1 g, α-naphthalene acetic acid 4 g. First, fully dissolve gibberellin and α-naphthylacetic acid with 200ml of acetone (the acetone will volatilize in the subsequent production process), then fully mix with about 4 kg of BSY2 mother powder, and then fully mix with thiophanate-methyl. Then mix with th...

Embodiment 2

[0044]The production formula of the seed coating agent in this embodiment adopts: 18.0% of BSY2 mother powder (50 billion cfu / gram of live spores, 200 meshes of fineness), 0.85% of iprodione, 0.003% of alpha-naphthalene acetic acid, 71.20% of bentonite, polyethylene Pyrrolidone 4.50%, sodium lignosulfonate 5.45%.

[0045] Accurately weigh 18.0 kg of BSY2 master powder, 0.85 kg of iprodione, 0.003 kg of α-naphthalene acetic acid, 71.20 kg of bentonite, 4.5 kg of polyvinylpyrrolidone, and 5.45 kg of sodium lignosulfonate.

[0046] First use 4 liters of absolute ethanol (ethanol will volatilize in the later production process, and the coated seeds do not contain or only contain a small amount of ethanol), fully dissolve 3 grams of α-naphthalene acetic acid and 850 grams of iprodione. Then fully mix the BSY2 master powder and bentonite, then add the above ethanol solution, and mix well again. Finally, polyvinylpyrrolidone and sodium lignosulfonate were added, fully mixed again, p...

Embodiment 3

[0049] The production formula of the seed coating agent in this embodiment adopts: 18.5% of BSY2 mother powder (the number of live spores is 100 billion cfu / gram, and the fineness is 120 mesh), 2.5% of the original drug of carbendazim (Nippon Soda Co., Ltd.), α-naphthaleneacetic acid 0.005%, diatomaceous earth 61.00%, sodium carboxymethylcellulose 14.50%, sodium lignosulfonate 3.5%.

[0050] Accurately weigh 18.5 kg of BSY2 master powder, 2.5 kg of carbendazim, 0.005 kg of α-naphthalene acetic acid, 61.00 kg of diatomaceous earth, 14.50 kg of sodium carboxymethyl cellulose, and 3.5 kg of sodium lignosulfonate.

[0051] Dissolve α-naphthalene acetic acid in 0.5 liters of absolute ethanol, mix well, then add it to 18.5 kg of BSY2 mother powder, mix well, add carbendazim, and mix evenly. Add sodium lignosulfonate, sodium carboxymethyl cellulose and diatomaceous earth in sequence and mix while adding, and finally pass through an airflow mill and pulverize to 325 mesh. The coating ...

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Abstract

The present invention provides a Bacillus subtilis, which is Bacillus subtilis BSY2. The Bacillus subtilis BSY2 is preserved in China Center for Type Culture Collection on June 20th, 2007, and the culture preservation number is CCTCC No.M207086. The present invention also provides a seed coating made from the Bacillus subtilis and an application thereof. The Bacillus subtilis is a strain screenedfrom the nature, which has outstanding prevention and curing effects on diseases in the seeding stage of cotton, and the inhibiting capability of the strain on cotton rhizoctoniosis, cotton anthracnose and cotton boll rot excels strains such as BS-208 and B-916. The seed coating made from the strain can be used to coat various cotton seeds and prevent and cure diseases in the seeding stage of cotton.

Description

technical field [0001] The invention relates to a bacillus subtilis, its seed coating agent and its use, belonging to biological pesticide products. Background technique [0002] Seed coating agent is a new pesticide formulation used for seed coating treatment. The seed coating agent is to adhere the active ingredients of pesticides to the periphery of the seeds, and then sow them into the soil with the seeds to play the role of disease resistance and insecticide, so as to achieve the purpose of uniform seedlings and strong seedlings. [0003] Seed coating technology includes film seed coating technology and pelletization technology. The film seed coating technology does not change the shape and size of the seeds, and the amount of pesticides is less. The pelletization technology will change the shape of the seed, increase the volume of the seed, and increase the weight of the seed. Since the pelletized seed coating technology needs to pack more substances on the outside ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01C1/06C12R1/125
Inventor 林开春罗振亚冯斌李芳张用梅黄昌华
Owner 武汉天惠生物工程有限公司
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