Numerator mark concatenated with rice purple pericladium gene PSH1 (t), acquiring method and application thereof

Abstract

The invention discloses a molecular marker of rice purple leaf sheath gene PSH1(t) linkage, and an acquired method and an application thereof, which belong to the field of molecular genetics. The rice non purple leaf sheath variety fragrant japonica 9407 and the purple leaf sheath variety R151 and the F2 which is obtained by hybridization are extracted with isolated population individual DNA. The two parents receives the PCR product polymorphism screening by utilizing 360 pairs of SSR molecular markers, then the screening of the molecular markers of purple leaf sheath gene linkage is carried out sequentially to a F2 mixing pool with purple leaf sheath DNA, a F2 mixing pool without purple leaf sheath DNA and each of the individuals of a F2 population, and the molecular genetic map of the purple leaf sheath gene PSH1(t) is constructed on the first chromosome. The selection efficiency of purple leaf sheath rice is greatly improved through the molecular markers of the major genes of purple leaf sheaths to test whether purple leaf sheath variety R151 and derived varieties (lines) thereof contain the genes of the purple leaf sheaths. The invention is specialized in the screening of the rice purple leaf sheath genes, and is used for the study of cloning the purple leaf sheath gene PSH1 (t).

Description

technical field [0001] The invention relates to a molecular marker linked with the rice purple leaf sheath gene PSH1(t) and its obtaining method and application, belonging to the field of molecular genetics. Background technique [0002] Rice is an important food crop in my country. form Traits play an important role in the genetic improvement and map construction of rice. In the current traditional breeding process, breeders still use easily identifiable morphological characters as markers to guide breeding. In recent years, with the development of hybrid rice in my country, the purity identification of hybrid rice seeds has become more and more important. Select leaf color marker traits that are obviously different from normal green leaves at the seedling stage, and the plants are robust, and have little impact on yield, such as purple leaves, purple leaf sheaths, and light green leaves, and introduce them into sterile lines and restorer lines. Helping to detect false...

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Problems solved by technology

Bing et al. (2006) used the recombinant inbred line population to locate three QTLs related to rice purple leaf sheath traits, which were located on rice chromosomes 1, 6, and 10, respectively, and located between the SSR markers RM129 and RM5 on chrom...

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