Anti-tumor metastasis medicament high flux screening model

An anti-tumor metastasis, high-throughput technology, applied in the direction of measurement devices, biochemical equipment and methods, microbial determination/testing, etc., can solve the problems of screening anti-tumor metastasis drugs, etc., and achieve the effect of high sensitivity and easy operation
CN101376907AInactive Publication Date: 2009-03-04CHINA PHARM UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA PHARM UNIV
Publication Date
2009-03-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of medicine and discloses an anti-tumor transfer medicine high throughput screening model. The screening method of the model comprises the following steps: an MDA-MB-435 cell is inoculated to a culture plate; a chemical compound to be screened is added into culture fluid and no inhibitor is added in; the chemical compound is cultured for an appropriate time of period and repeatedly melted, to obtain cell lysis solution; the cell lysis solution is added into human thrombinogen compound solution to be incubated together; a chromogenic substrate is added in; at 405nm, absorbency is determined and inhibitory rate is calculated; the chemical compound with the inhibitory rate higher than 20 percent is a positive compound. In the invention, the human breast cancer cell MDA-MB-435 is selected to build the anti-tumor transfer medicine screening model which is quick and stable, has high sensitivity and is convenient to be operated; the model is characterized by trace quantity and accuracy, and is applicable in screening anti-tumor transfer medicine with high throughput.
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Description

technical field

[0001] The invention belongs to the field of medicine and relates to a high-throughput screening model of anti-tumor metastasis medicine. Background technique

[0002] Tissue factor (TF for short), that is, coagulation factor III, is a single-chain transmembrane glycoprotein, a member of the cytokine superfamily, and is the initiating factor of physiological hemostasis, coagulation process and various pathological thrombus formation. [1] . TF is both a cell surface receptor for coagulation factor VII (FVII) and a cofactor for FVII or FVII(a). When the blood vessel is damaged or under pathological conditions, the blood is exposed to the surface of cells expressing TF, and the receptor TF forms a complex with its ligand VII or VIIa, thereby simultaneously activating blood coagulation factors X and IX, and the activated FXa and FIXa are further activated Prothrombin (FII), FIIa turns fibrinogen into fibrin, thereby completing the coagulation cascade, leading t...

Claims

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