Nitroso mercaptan fluorescent quantitative determination kit
A fluorescence quantitative detection and nitrosothiol technology, which is applied in the direction of fluorescence/phosphorescence, chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, so as to save the experimenter's time and simplify the complicated steps.
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[0016] Below in conjunction with accompanying drawing, the composition of the present invention and using method are described in further detail:
[0017] The composition of the detection kit:
[0018] Reagent bottle 1: 1.5mL EP tube packed in a black plastic bag, and DAN solution dissolved in 0.62M HCl is sealed inside the tube. Accurately weigh 0.05mg DAN, fully dissolve in 1mL 0.62M HCl solution, this is reagent I.
[0019] Reagent bottle 2: A 1.5mL EP tube packed in a black plastic bag, and the inside of the tube is sealed and packed with a DAN solution of 0.62M HCl containing 3.7mM mercuric chloride. Accurately weigh 0.001g of mercuric chloride, dissolve it in 20μL DMSO (dimethyl sulfoxide), and dissolve it together with 0.05mgDAN in 1mL of 0.62M HCl solution. This is reagent II.
[0020] Reagent bottle 3: white plastic bottle, sealed with NaNO inside the bottle 2 standard solution. Accurately weigh 0.0069g NaNO 2 , dissolved in 10 mL double-distilled water to obtain...
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