Method for measuring hydroxy free radical

A determination method and free radical technology, which can be used in material analysis by observing the influence of chemical indicators, and by chemical reaction of materials. Simple, accurate results

Inactive Publication Date: 2009-04-22
UNIV OF SHANGHAI FOR SCI & TECH
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AI Technical Summary

Problems solved by technology

These methods or instruments are expensive or not sensitive enough
[0003] There is no spectrophotometric method for the determination of hydroxyl radicals using naphthyl diphenylmethane dye-Victoria Brilliant Blue BO (C.I 42595) as a chromogenic agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] All reagents used were of analytical grade.

[0019] Drawing of standard working curve

[0020] Shift 1.0×10 -2 mol L -1 Potassium iodide aqueous solution 8.0mL, 1.0×10 -3 mol L -1 FeSo 4 0.1mL in a 50mL volumetric flask, take the prepared 5.0×10 -4 mol L-1 hydrogen peroxide 0.2, 0.4, 0.6, 0.8, 1.0mL were poured into the volumetric flask respectively, and then 8.0mL of phosphoric acid-sodium dihydrogen phosphate buffer solution with pH 2.0, emulsifier OP0.5g L -1 2.0×10 -4 mol L -1 Victoria Brilliant Blue BO aqueous solution 10.0mL, constant volume with distilled water, shake well, let stand for 15min, use 1cm cuvette, reagent blank as reference, measure the absorbance of the solution at 571nm, take the absorbance as the ordinate, and take the hydrogen peroxide concentration As the abscissa, draw a standard curve.

[0021] Obtain the regression equation according to the standard curve: A=0.06C (mol L -1 )+0.002(r=0.9994, 0.0mg·L -1 ~0.0082mol·L -1 ); Wherein...

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Abstract

The invention relates to a hydroxyl free radical testing method, in particular to a spectrophotometry for testing the hydroxyl free radical by using naphthyl diphenyl methane dye-victoria pure blue BO(C.I42595) as a chromogenic reagent. 8.0ml of 1.0 multiplied by10<-2>mol.L<-1> potassium iodide solution, 0.1ml of 1.0 multiplied by 10<-3> mol.L<-1> FeSO4, a tested object which can produce the hydroxyl free radical, 8.0ml of phosphoric acid-dihydric phosphate buffer solution with PH value of 2.0, and , 10.0 ml of 2.0*10<-4>mol.L<-1> of victoria pure blue BO aqueous solution with 0.5g.L<-1> of emulsifier OP are added sequentially into a volumetric flask with capacity of 50ml, then carries out the constant volume by distilled water, are evenly shaken, and stands for 15 minute, and then a 1cm colorimetric ware is taken as reference, the absorbency of the solution is tested at the distance of 571nm, and finally, a regression equation is obtained according to a standard curve: A is equal to 0.06C(mol.L<-1>) plus 0.002(r is equal to 0.9994, 0.0mg.L<-1> to 0.0082mol.L<-1>, wherein, A stands for absorbency; C stands for molar concentration of the hydroxyl free radical, i.e. molar concentration of hydrogen peroxide), and the concentration of hydroxyl free radical can be calculated. The method of the invention has the advantages of simple instruments, simple and convenient operation, high sensitivity and accurate analyzing result.

Description

technical field [0001] The present invention relates to a method for measuring hydroxyl radicals, in particular to a method for determining hydroxyl radicals by spectrophotometry using naphthyl diphenylmethane dye—Victoria Brilliant Blue BO (C.I 42595) as a color developer. Background technique [0002] Hydroxyl radicals (·OH) are highly toxic and harmful to organisms, but they have high reactivity, short lifespan and low concentration. At present, the methods reported at home and abroad for the determination of hydroxyl radicals include: spectrophotometry, electron spin resonance method, high performance liquid chromatography, fluorescence method, resonance scattering spectroscopy and so on. These methods or instruments are expensive, or the sensitivity is not enough. [0003] There is no method for the spectrophotometric determination of hydroxyl radicals using naphthyl diphenylmethane dye—Victoria Brilliant Blue BO (C.I 42595) as a chromogenic agent. Contents of the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 施文健秦琴闻海峰张元璋王亚伟陈树薇陈肖云钟晓永高隽臣杨琴淋
Owner UNIV OF SHANGHAI FOR SCI & TECH
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