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Goat pox vaccine strain expression vector

An expression vector, goat pox virus technology, applied in biochemical equipment and methods, applications, botany equipment and methods, etc., can solve problems such as spending more time and high cost

Inactive Publication Date: 2009-04-29
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to prevent the occurrence of sheeppox, sheep in this area must be immunized with live sheeppox vaccine at least once a year, and with the immunization of other diseases, sheep epidemic prevention will take more time and cost more

Method used

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  • Goat pox vaccine strain expression vector
  • Goat pox vaccine strain expression vector
  • Goat pox vaccine strain expression vector

Examples

Experimental program
Comparison scheme
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Embodiment

[0031] Describe the example of the present invention below by the construction of expression vector and application method

[0032] (1) Basic method of DNA recombination

[0033] 1. Preparation and Transformation of E. coli Peptide Sensitive Cells

[0034] The preparation of Escherichia coli competent cells is prepared by calcium chloride method. The specific method refers to Sambrook J, Russell D W. Translated by Huang Peitang, Wang Jiaxi, Zhu Houchu, etc., >. 3rd Edition Translated, Beijing: Science Press, 2002: 96-98. Use 0.1mol / L mgcl2 and 0.1mol / L CaCl2. Wash by mixing 4:1. For the transformation method of competent cells, refer to the above-mentioned references.

[0035] 2. Mini-preparation of plasmids (unless otherwise specified, the configuration of reagents refers to "Molecular Cloning Experiment Guide". 3rd edition translation, Beijing: Science Press, 2002)

[0036] Refer to "Molecular Cloning Experiment Guide" translated by Huang Peitang et al. 3rd edition tran...

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Abstract

The invention provides an expression vector used for expressing foreign protein in the recombination of goat pox virus. A foreign gene is inserted in the down stream of a VVI1L promoter or a VVP11 promoter in the expression vector, by the homologous recombination with the parent plant of the goat pox virus, 12 recombined sheep pox viruses for expressing the foreign protein are obtained, a flanking sequence in the vector, which can make the homologous recombination with the parent plant of the goat pox virus, is a sheep pox virus G14-STV-44-55 vaccine strain which contains TK gene, IFNR-gamma gene or RR small subunit gene, by utilizing the expression vectors, the recombined goat pox viruses expressing different foreign proteins can be constructed by different methods, and the recombined goat pox viruses can be used as recombined live vector vaccines to prevent and treat diseases and study relations of expression, processing, structure and function of different biological active proteins.

Description

Technical field: [0001] The invention provides an expression vector used for expressing foreign protein in recombinant goat pox virus, which belongs to the field of biotechnology. Background technique: [0002] The development of modern molecular biology has enabled people to manipulate the genes of various organisms, mastered the technology of cloning and recombining DNA in bacterial plasmids, thus greatly expanding the methods for people to make vaccines, and developed a variety of genetic vaccines to prevent and control animals. blight, viral vector live vaccines are one of them. [0003] The ability of poxviruses to provide strong immune protection was deeply understood long ago during the fight against smallpox. Learn that poxviruses are the largest of animal viruses and can be seen under a light microscope. Under an electron microscope, the virus has a brick-shaped appearance. The viral genome is composed of single-molecule double-stranded DNA, with a G+C content of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/863A61K48/00
Inventor 陈创夫郭志儒李有文魏风
Owner SHIHEZI UNIVERSITY
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