Gene order relating to cordycepin biological synthesis

Abstract

The invention relates to cordycepin biosynthetic related genes and application thereof. By adopting a suppression subtractive hybridization (SSH) technique, seven cordycepin biosynthetic related genes which are named as chcs1, chcs2, chcs3, chcs4, chcs5, chcs6 and chcs7 respectively are cloned, wherein the length of cDNA sequence of the chcs2 is 774bp. Through constructing an expression transformative vector of a high-efficiency plant containing the genes, means of biosynthetic gene copy amount in thalli is increased, and the content of cordycepin thalli for expressing cordycepin is improved, so as to meet requirement on medical development.

Description

[0001] This application is a divisional application with the filing date of September 06, 2005, the application number of 200510037011.8, and the title of "gene sequence related to the biosynthesis of cordycepin". technical field [0002] The invention relates to a secondary metabolite biosynthesis gene of the Cordyceps fungus in the field of bioengineering, in particular to a gene related to the biosynthesis of cordycepin. Background technique [0003] Cordyceps fungi are an important class of entomogenous fungi. Cordycepin is one of the important active ingredients of Cordyceps sinensis and Cordyceps militaris. It has unique effects in antibacterial, antiviral, antitumor, insecticidal and other aspects. Development of valuable nucleoside antibiotics. More than 100 documents have been published abroad to introduce the research on anti-tumor, anti-virus, anti-bacterial and extraction and purification of cordycepin. [0004] In 1951, Americans such as Cuningham (Cuningham) ...

AI Technical Summary

Problems solved by technology

Its shortcoming is that the conventional traditional method mentioned above has a long cycle and unsatisfactory incremental effect

Images