Method for extracting anti-tumor effective component of Prunella vulgaris and application of extract thereof in preparing anti-tumor medicament

Abstract

The invention relates to a method for extracting antitumor effective sites of selfheal, which comprises: using ethanol to soak spica of the selfheal in an extractor, and performing heating, reflux and extraction; concentrating an extracting solution into a dried extract, using chloroform and water or ethyl acetate and the water to extract the dried extract, respectively collecting an organic phase and an aqueous phase, and concentrating the organic phase and the aqueous phase to be dried; using the chloroform, methanol and the water to dissolve the organic phase, separating out superstratum after standing, and concentrating an upper solution to be dried; and adding a mobile phase to dissolve upper residue, performing reversed-phase column chromatography and elution, collecting 30 to 80 percent methanol eluent, concentrating eluent of various phases to be dried, and obtaining the antitumor effective sites. The in vitro experiments of the effective sites of the selfheal determined by the proposal of the method can obviously inhibit proliferation of Rajicells, Jurkat cells, K562 cells and 435S cells; and the in vivo experiments have tumor inhibitory efficacy on a He T lymphoma EL-4 cell mouse, and can prolong the life cycle of the He T lymphoma EL-4 cell mouse; and the antitumor effective sites of the selfheal have definite antitumor function and low toxicity.

Description

Extraction method of anti-tumor effective part of Prunella vulgaris and application of its extract in preparation of anti-tumor drugs technical field The invention relates to a method for extracting the anti-tumor effective part of Prunella vulgaris and its anti-tumor effect. Background technique Prunella vulgaris is the dry and mature ear of Prunella vulgaris L, a plant of the Labiatae family. It is mainly produced in Queshan County, Henan Province and other places. disease. Prunella vulgaris has a long history of treating malignant lymphoma. Many folk prescriptions and proven prescriptions use Prunella vulgaris as the main drug for treating malignant lymphoma. According to the clinical observation of treating malignant lymphoma with the compound traditional Chinese medicine preparation with Prunella vulgaris as the main medicine for many years, the applicant of the patent found that it can make the tumor body of malignant lymphoma subside. Modern pharmacological studie...

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Problems solved by technology

According to the literature search and patent search, there is no experimental research report on the effective extraction parts of Prunella vulgaris against malignant lymphoma

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