Classification and production technique of newborn calf serum

A technology of newborn bovine serum and production process, which is applied in the field of classification and production technology of newborn bovine serum, and can solve the problems of unseen newborn bovine serum classification, waste, cost increase and the like.

Inactive Publication Date: 2011-09-28
INST OF BASIC MEDICINE OF SAMS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are available data on using A549 cells as the attachment efficiency and SP2 / 0 cells as the cloning efficiency to measure the quality of serum, no one uses different cell lineages for cell proliferation tests or attachment tests for different production purposes, nor See information and products on Serum Antibody Detection and Classification of Newborn Cattle Serum for Production Purposes
This forces vaccine manufacturers to run a serum screening program first before producing vaccines, resulting in increased costs and waste

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  • Classification and production technique of newborn calf serum
  • Classification and production technique of newborn calf serum
  • Classification and production technique of newborn calf serum

Examples

Experimental program
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Embodiment 1

[0054] Example 1: The specific use of different vaccines in animal husbandry for the production of newborn bovine sera by classification, the steps are as follows:

[0055] 1 Raw material newborn bovine serum: newborn bovine serum comes from 50 newborn calves, number: 200800001~200800050, and each raw material newborn bovine serum has 5 small samples to be tested.

[0056] 2 Detection of raw material newborn bovine serum:

[0057] 2.1 Microbiological testing:

[0058] 2.1.1 Bacterial detection: Inoculate 10 reserved samples with more serum samples ① respectively inoculate 2 copies of broth liquid culture medium and culture them at 37°C and 25°C for 7-15 days, observe the turbidity of the medium, and do Gram staining on the smear , Under the microscope, look for regular, microbial-like shapes such as rods or spheres. At the same time, 10 reserved samples ① were inoculated with fungal solid culture, cultured at 37°C and 25°C for 10-20 days, and the growth of fungal colonies wa...

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Abstract

The invention discloses a classification and production technique for new-born calf serum. Albumen, hemoglobin and bacterial endotoxin quantitative determination, microbiology determination and antibody determination are carried out on the new-born calf serum; then new-born calf serums with identical backgrounds are classified and amalgamated; cell proliferation spectral test and basic quality control are carried out on the classified and amalgamated new-born calf serums; the cell proliferation spectral test consists of continous cell culture proliferation test and primary cell culture test; Finally, according to cell proliferation and antibody background, the usages of the serums are classified. The calf serums produced by the technique of the invention has clear classification and exclusive usages, thus meeting the requirement that the production of different vaccines of biomedicine needs different serums, improving the quality of the vaccines and reducing the production cost.

Description

technical field [0001] The invention relates to a classification production process of newborn bovine serum. Background technique [0002] At present, bovine serum in the domestic market is produced in accordance with national standards. [0003] The national standard for newborn bovine serum products meets the following requirements: 1: Newborn bovine serum is divided into top grade, super grade, special grade and Common level 4; 2: The total protein content is specified to be 3.5-5.0 (W / V,) hemoglobin content ≤ 0.035 (W / V), endotoxin content ≤ 10EU / ml, negative sterility test, negative mycoplasma and coliphage negative ; 3: Negative for bovine diarrhea virus antibody and foot-and-mouth disease antibody; 4: The serum promotes the growth of BHK cells and SP2 / 0 cells (see Table 12 for details). [0004] The inventor of the present application found in the process of supplying newborn bovine serum products to various animal husbandry vaccine production units that producing d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61K35/16
Inventor 温晓燕孟红李鹏岳盈盈李志会宋楠楠
Owner INST OF BASIC MEDICINE OF SAMS
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