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Tobacco black shank resistance disease nursery identification method

A technology of tobacco black shank and an identification method, which is applied in the field of plant disease resistance identification, can solve the problems of high cost, limited number of races, limited variety of species, and high operation technology, and achieves low cost, good natural irrigation conditions, representative effect

Active Publication Date: 2009-07-29
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The shortcoming of current identification method is that the collection, preservation and reasonable selection of bacterial strains for inoculation are long-term, continuous, high-tech, and costly work, and the number of races and species diversity used for inoculation are not consistent with field test methods. is always finite compared to

Method used

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  • Tobacco black shank resistance disease nursery identification method
  • Tobacco black shank resistance disease nursery identification method
  • Tobacco black shank resistance disease nursery identification method

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Experimental program
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Effect test

Embodiment 1

[0017] 1.1 Site selection and establishment of naturally induced disease nurseries in the field

[0018] The geographical and ecological environment that is conducive to the onset and stability of the onset is the basis of the disease nursery. The pathogen survives the winter in diseased plant residues, soil, and manure with chlamydospores and dormant mycelium, and can survive for about 3 years. Bacteria-carrying soil, fertilizers, irrigation water and diseased seedlings are the main sources of primary infection of tobacco black shank. Bacteria are repeatedly re-infected by wind and rain, irrigation water, agricultural operations, etc., making the disease more and more serious, and then spreading to the whole field. Tobacco black shank bacteria mainly concentrated in the soil layer 0-5cm below the soil surface, invaded through wounds or directly, the infection site was mainly at the base of the stem, and the infection stage was mainly before budding. High temperature and hig...

Embodiment 2

[0080] Identification of tobacco germplasm resources against black shank

[0081] Tobacco black shank is a worldwide devastating fungal disease of tobacco caused by Phytophthora parasitica var.nicotianae (Breda deHean) Tucker. The pathogen belongs to the flagellate subphylum Phytophthora genus, is a facultative parasite that likes high temperature and high humidity, can harm all types of tobacco, and can infect roots, stems, and leaves at any stage of tobacco growth, causing wilting, stem damage and Plants die. In recent years, due to the expansion of the area of ​​continuous cropping tobacco fields and the increase of continuous cropping years, tobacco black shank has become more prevalent. In order to reduce the losses caused by the disease, people have used various methods such as agricultural control, chemical control, and disease-resistant breeding to control tobacco black shank disease. The post-growth period is short, the onset is fast, and multiple re-infections can ...

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Abstract

The invention relates to a method for identifying tobacco black shank resistance in disease nursery. A continuous cropping tobacco field in a historical high occurrence area of the tobacco black shank disease is selected as a disease nursery; plants are inducted to develop the disease through the improvement of the field humidity, so that the complex operation of strain separated storage and culture is reduced; the strain is sourced from the field; the quantity and variety of the microspecies for inoculation are theoretically unlimited; and the method has better diversity and representativeness, and the inoculation conditions are under the control of nature, so the identification results reflect the local practical situation of the day more correctly. A one-off uprooting investigation method after the collection of the flue-cured tobacco is adopted so as to greatly reduce the workload, save the cost and bring about a correct and reliable result. The method is characterized by low cost and simple and feasible operation. The method is an economical and practical method and has bright market and application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance identification, in particular relates to the identification of tobacco black shank resistance, in particular to a method for identifying tobacco black shank disease resistance. Background technique [0002] Tobacco black shank is one of the most serious diseases of tobacco in the world, and it is also the main disease of tobacco in my country. It can harm all cultivated tobaccos such as flue-cured tobacco, air-cured tobacco, sun-cured tobacco, burley tobacco, and oriental tobacco. Tobacco leaves lost due to tobacco black shank accounted for about 1% to 2% of the total output in North Carolina, USA. In 1995 and 1996, the losses caused by black shank reached 36.1 million US dollars, accounting for the total loss of the disease. 35.13%, ranking first among all diseases. The disease has a wide distribution in my country, except Heilongjiang Province, which has not yet been found, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01G1/00
Inventor 李梅云李永平刘勇卢秀萍高家合
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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