Method for preparing natural antioxidative food additive
A technology for oxidizing food and additives, applied in the fields of food preservation, food science, application, etc., can solve problems such as anxiety, and achieve the effect of comprehensive and stable antioxidant capacity
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Embodiment 1
[0019] A preparation method of a natural antioxidant food additive, the specific steps are:
[0020] Step 1: Cultivate Hansenula vietinosa, Monascus purpura, Acetobacter pasteurianus and Lactobacillus bulgaricus on eggplant bottles at 30°C for 45 hours, and store them at 2°C for later use;
[0021] Step 2: Inoculate Hansenula viticola, Monascus purpura, Acetobacter pasteurianus and Lactobacillus bulgaricus obtained in the first step into LB culture medium respectively, and Hansenula viticola, Monascus purpura, Acetobacter pasteurii and Lactobacillus bulgaricus respectively The weight ratio of the bacilli to the LB culture solution is 25:5:10:20:100, cultured at 30° C. for 6 hours in a shaker flask;
[0022] Step 3: Sterilize the fermentation tank with steam at a temperature of 120° C. and a pressure of 0.142 Mpa for 25 minutes, add the fermentation base material, and pasteurize it at 70° C. for 10 minutes. The fermentation base material consists of the following in parts by we...
Embodiment 2
[0030] A preparation method of a natural antioxidant food additive, the specific steps are:
[0031] Step 1: Cultivate Hansenula vietinosa, Monascus purpura, Acetobacter pasteurianus and Lactobacillus bulgaricus on eggplant bottles at 40°C for 50 hours on their respective medium, and store them at 6°C for later use;
[0032] Step 2: Inoculate Hansenula viticola, Monascus purpura, Acetobacter pasteurianus and Lactobacillus bulgaricus obtained in the first step into LB culture medium respectively, and Hansenula viticola, Monascus purpura, Acetobacter pasteurii and Lactobacillus bulgaricus respectively The weight ratio of the bacillus to the LB culture solution is 30:10:15:30:100, cultured at 40°C for 10 hours in a shaker flask;
[0033]Step 3: Sterilize the fermenter with steam at a temperature of 125° C. and a pressure of 0.152 Mpa for 35 minutes, add the fermentation base material, and pasteurize it at 80° C. for 20 minutes. The fermentation base material consists of the follo...
Embodiment 3
[0041] A preparation method of a natural antioxidant food additive, the specific steps are:
[0042] Step 1: Cultivate Hansenula vietinosa, Monascus purpura, Acetobacter pasteurianus and Lactobacillus bulgaricus on eggplant bottles at 35°C for 47 hours, and store them at 4°C for later use;
[0043] Step 2: Inoculate Hansenula viticola, Monascus purpura, Acetobacter pasteurianus and Lactobacillus bulgaricus obtained in the first step into LB culture medium respectively, and Hansenula viticola, Monascus purpura, Acetobacter pasteurii and Lactobacillus bulgaricus respectively The weight ratio of the bacilli to the LB culture solution is 27:7:12:25:100, cultured at 35° C. for 8 hours in a shaker flask;
[0044] Step 3: Sterilize the fermenter with steam at a temperature of 121° C. and a pressure of 0.148 Mpa for 30 minutes, add the fermentation base material, and pasteurize it at 75° C. for 15 minutes. The fermentation base material consists of the following in parts by weight Co...
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