Micro-fluidic chip detecting system using quartz crystal microbalance as transducer

A microfluidic chip, quartz crystal technology, applied in the direction of determination/inspection of microorganisms, instruments, measuring devices, etc., can solve the problems of time-consuming and laborious, complex detection devices and sample labeling, cumbersome steps, etc., and achieve simple, fast and convenient operation. Detection of biomolecules, low maintenance and repair costs

Inactive Publication Date: 2009-09-30
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far there is no instrument that combines the two instruments to detect biomolecules
Liu Tao and others used QCM and Eileen T.Dimalanta and others used microfluidic chips to detect DNA. The former relies on manual sample loading steps, which are cumbersome and time-consuming, while the latter requires complex detection devices and sample labeling.

Method used

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  • Micro-fluidic chip detecting system using quartz crystal microbalance as transducer
  • Micro-fluidic chip detecting system using quartz crystal microbalance as transducer
  • Micro-fluidic chip detecting system using quartz crystal microbalance as transducer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] See figure 1 The connection method of the system: place the precision constant temperature chamber 12 with the sample inlet and the cleaning port on the shockproof and antistatic working platform 1, and the precise constant temperature chamber is installed with a movement accuracy of 0.1mm with X, Y, and Z directions. The three-dimensional mobile device 2 with X, Y, and Z directions is fixed with a QCM 3 with a frequency of 9 MHz (QCM is composed of gold sheets and crystals, products of Chenjing Electronics Co., Ltd., Beijing), and is installed above the QCM There is a microfluidic chip 4 with a microvalve, and the bottom of the microfluidic chip with a microvalve has a sample outlet; the three-dimensional moving device with X, Y, and Z directions ensures that the center of the QCM is aligned with the microfluidic chip with a microvalve. The sample port of the microfluidic chip is accurately docked.

[0053] The microfluidic chip with microvalve is a sandwich structure...

Embodiment 2

[0068] The system of Example 1 and the microfluidic chip with microvalve and probe and target DNA were used.

[0069] Pre-heated at 60°C with a mass concentration of 98% H at a volume ratio of 3:1 2 SO 4 with a mass concentration of 30% H 2 o 2 The QCM cleaned by the mixed solution was soaked in HS(CH 2 ) 6 In SH and ethanol solution, HS(CH 2 ) 6 SH is adsorbed on the QCM through the Au-SH bond, and after 30 minutes, a 12V micro-water pump is used to draw secondary water for washing, and N 2 Blow dry (provided by an air tube connected to the air intake device above the edge of the QCM); then modify the HS (CH 2 ) 6 The SH QCM was immersed in a solution containing gold nanoparticles with a diameter of 12 nm, which were adsorbed on HS (CH 2 ) 6 On SH, after 30 minutes, use a 12V micro-pump to extract secondary water for flushing, N 2 Blow dry, and record frequency 3 at this time. will be modified with HS(CH 2 ) 6 The QCM of SH and gold nanoparticles is fixed on a t...

Embodiment 3

[0071] The system of Example 1 and the microfluidic chip with microvalve are used.

[0072] Pre-heated at 60°C with a mass concentration of 98% H at a volume ratio of 3:1 2 SO 4 with a mass concentration of 30% H 2 o 2 The mass concentration of QCM immersed in the cleaned mixed solution is 1mg mL -1 In the solution of avidin, avidin is adsorbed on the QCM through chemical bonds, washed with water after 30 minutes, N 2 Dry air (provided by a trachea connected to the air intake device above the edge of the QCM), record the frequency 5 at this time; fix the avidin-modified QCM on a three-dimensional mobile device with X, Y, and Z directions, and use it to The movement enables the precise docking between the QCM center and the microfluidic chip outlet d, and 30 μL is added to the microfluidic chip inlet b with a concentration of 2.0×10 -6 M / L biotin (biotin) is connected to the target biotin-TAR RNA of TAR RNA, close the injection port a and c, and enter the N in the gas chan...

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Abstract

The invention relates to a biological molecule detecting system combing a micro-fluidic chip with a micro-valve and quartz crystal microbalance (QCM). The system is characterized in that a shock-proof and ant-static platform is provided with the micro-fluidic chip with the micro-valve and a precise thermostatic chamber connected with the QCM; a sample in the micro-fluidic chip controls the micro-valve to open and close in an intermittent mode through airflow formed by a vacuum pump and an air inlet system controlled by an electromagnetic valve; the QCM is connected with a data collecting device through a frequency collector and arranged on a three-dimensional moving device with X, Y and Z directions; the center of the QCM is precisely abutted with a sample outlet of the micro-fluidic chip with the micro-valve for sampling; after the adsorption of the sample, a minitype water pump sweeps non-adsorbed sample, an air inlet device blows and dries the sample, and the QCM changes the frequency to detect certain biological molecules; the data collecting device is connected with the frequency collector, the precise thermostatic chamber, the electromagnetic valve control device and the three-dimensional moving device with X, Y and Z directions. The system has the functions of preprocessing sample and fast and conveniently detecting biological molecules.

Description

technical field [0001] The invention relates to a biomolecule detection system combining a microfluidic chip with a microvalve and a quartz crystal microbalance (QCM). Specifically, the present invention provides a microfluidic chip detection system that can be used for rapid detection of biomolecules and uses a quartz crystal microbalance as a transducer. The system has the functions of sample pretreatment and fast and convenient detection of biomolecules. The operation is simple, and the cost of manufacture, use, maintenance and repair is relatively low. Background technique [0002] Quartz crystal microbalance (QCM) is a sensor that detects trace substances based on the change of electrical signal caused by the quality of the substance. It has the advantages of high detection sensitivity, easy to adjust selectivity, low cost, especially timely detection without sample marking, etc. . It is a broad-spectrum detector, which can be used to test the immobilization of biolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N5/00C12Q1/00
Inventor 李兴长李少华张建平张雅坤刘涛江龙
Owner INST OF CHEM CHINESE ACAD OF SCI
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