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Cell line for lowly-metastatic clear cell carcinoma of kidney of Han Chinese

A transparent cell and transferable technology, applied in artificial cell constructs, animal cells, vertebrate cells, etc., can solve the problems of lack of systematic and in-depth forward-looking basic theoretical research

Inactive Publication Date: 2011-06-29
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, research on renal cell carcinoma mainly focuses on the improvement of diagnostic methods, selection of treatment options, and retrospective pathological analysis, lacking systematic and in-depth prospective basic theoretical research

Method used

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  • Cell line for lowly-metastatic clear cell carcinoma of kidney of Han Chinese
  • Cell line for lowly-metastatic clear cell carcinoma of kidney of Han Chinese
  • Cell line for lowly-metastatic clear cell carcinoma of kidney of Han Chinese

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Obtaining of LoMet-ccRCC cell line of low metastatic renal clear cell carcinoma of Han nationality

[0037] The LoMet-ccRCC cells of the present invention are taken from the renal orthotopic tumor tissue of a Han patient with renal clear cell carcinoma. Put the excised tumor tissue into a glass dish containing 5ml D-Hanks solution, and remove necrotic tissue, fat tissue, blood vessels, etc. Soak the tissue block in 5-10ml 1640 medium for 30-40min, and cut the tissue block into 1-3mm 3 Transfer the soaking solution and tissue block to the centrifuge tube, shake and wash repeatedly for 2-3min, 1500rpm, centrifuge for 10min, discard the supernatant, re-add 5-10ml suspended tissue block of 1640 medium, and wash repeatedly Centrifuge for 10 minutes at 1500 rpm for 2-3 min, discard the supernatant, suspend the tissue block with 3-4 ml of 1640 complete medium, and transfer the suspension to the cell culture flask at 1 ml / bottle, and place the flask at 37°C , 5% CO 2 , ...

Embodiment 2

[0038] Example 2 LoMet-ccRCC cell growth curve

[0039] 3×10 4 A total of 21 cells were inoculated into a 24-well plate with 2ml of 1640 complete medium. After that, 3 wells were digested every other day and the cells were counted. Calculate the average and standard deviation of the number of cells, and draw a cell growth curve. Such as figure 2 As shown, the growth of LoMet-ccRCC cells was relatively stable, entering exponential growth on the 3rd day of inoculation, the growth rate reached the fastest on the 4th day, and then gradually slowed down. The cell doubling time was 16.5h.

Embodiment 3

[0040] Example 3 LoMet-ccRCC cell chromosome analysis

[0041] Counting the chromosomes of 100 cells in the metaphase of division, the number of chromosomes of the LoMet-ccRCC cell line is mainly concentrated between 50 and 70, and the chromosome mode is 55. Prompt the existence of hyperdiploid and so on.

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Abstract

The invention discloses a cell line for lowly-metastatic clear cell carcinoma of kidney of Han Chinese, namely LoMeT-ccRcc CCTCC No.C200910. The cell line can grow for a long time in vitro and be stably handed down, has epithelioid cell form, and has no contact inhibition. The cell is heteroploid, chromosome number and structure are aberrant. Immunohistochemistry shows masculine CA9 and CD133. A flow cytometry analyses and finds out that CA9 and CD133 expression is obviously enhanced in the condition that cell is handed down for many times. The cell line has lowly-metastatic trend after the cell is handed down continuously. The recovery rate of the cell line after being frozen is more than 80%, the recovered cell growth state accords with the original cell. The cell line of the invention is used for establishing a cell model for sieving and preparing medicines that is used for dialogising, preventing and treating clear cell carcinoma of kidney.

Description

Technical field [0001] The invention relates to a microbial animal cell line, in particular to a Han nationality human low-metastatic renal clear cell carcinoma cell line LoMet-ccRCC, CCTCC-C200910. Background technique [0002] Tumor cell lines play an important role in the basic research of tumors. The use of cell lines for in vitro experiments and the construction of in vivo animal models has become a research hotspot. The research results obtained are comparable and convincing. At present, the in vitro culture of human cancer cells has become a commonly used method in medical and biological research. It can not only study the mechanism of tumorigenesis and development at the molecular and genetic level, but also has important significance for clinical diagnosis, drug screening and tumor treatment. [0003] Renal cell carcinoma is the most common renal solid malignant tumor (about 96%). It ranks second in genitourinary system tumors, accounting for 3% of all adult tumors, and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12R1/91
Inventor 曹广文翟羽佳苏彤贺松琴常文军
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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