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Micro ribonucleic acid and application thereof

A technology of small ribonucleic acid and nucleic acid, applied in DNA/RNA fragments, measurement/inspection of microorganisms, recombinant DNA technology, etc., can solve problems such as tumor occurrence

Inactive Publication Date: 2009-10-14
SHANGHAI INST OF ONCOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current studies have shown that many miRNAs are involved in biological processes such as development, differentiation, growth, and immune response, and their expression and dysfunction may lead to various pathological phenomena such as tumorigenesis, leukemia, and viral infection.

Method used

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  • Micro ribonucleic acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example of Liver Cancer Prognosis Verification

[0055] Materials and Methods

[0056] Case Organization:

[0057] 78 pairs of cancerous and adjacent tissues were obtained from surgical resection specimens of patients with primary liver cancer. These specimens were obtained from the Department of Pathology of the First Affiliated Hospital of Zhejiang University and the Institute of Liver Cancer. 10 normal livers were obtained from accidental deaths. All tissue specimens obtained were approved by the ethics committee of the World Health Organization Collaborating Organization authorized by the Shanghai Municipal Government. All surgically resected specimens were pathologically analyzed, and the pathological grading was in accordance with Edmonson's criteria. Clinical data included: age, gender, tumor size, pathological grade, lymph node metastasis, liver cirrhosis status, HBV and HCV infection, and these data were obtained from the patient's case records. All patient...

Embodiment 2

[0071] Liver cancer prognosis detection kit

[0072] As described in Example 1, the small RNA represented by the sequence of SEQ ID NO: 1 is closely related to the prognosis of liver cancer. Therefore, detection can be performed based on this extracted patient's miRNA.

[0073] A test group consisting of 30 individuals was randomly selected, including subjects with unknown prognosis of liver cancer and patients with known prognosis of liver cancer.

[0074] Obtain liver tissue sections of the subjects to be tested in the test group, extract RNA and prepare test samples using the method as in Example 1.

[0075] RNA samples were obtained from Applied Biosystems The MicroRNA Reverse Transcription Kit uses specific primers with neck loops for reverse transcription to ensure that only mature miRNAs are detected during real-time PCR. 1-10 ng RNA was used for each reverse transcription reaction, and the target gene and U43 were reverse transcribed in the same reaction system. T...

Embodiment 3

[0094] Auxiliary test for prognosis of liver cancer

[0095] The detection in Example 2 was repeated, except that 10 individuals were randomly selected (the prognosis of liver cancer was not known before the detection) for detection.

[0096] The results also confirmed that the subjects with higher expression of SEQ ID NO: 1 had significantly longer survival time after liver cancer surgery than those with low expression.

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PUM

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Abstract

The invention discloses a specific expression micro ribonucleic acid relevant to liver cancer prognosis and an application of the same, for example, a probe specific to the micro ribonucleic acid and auxiliary test reagent or test kit which are used for detecting liver cancer prognosis, and the like.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a small RNA and related applications. Background technique [0002] Liver cancer is one of the most common tumors in Southeast Asia and South Africa, and its mortality rate is also very high. Many human diseases are caused by disordered or uncontrolled gene expression. Small RNAs (small RNAs, microRNAs, miRNAs) are a class of single-stranded small RNAs with a length of about 21-25nt that do not encode proteins. They are widely present in eukaryotes and bind to specific target mRNAs through nucleic acid sequence complementarity. It regulates the translation of target mRNA or degrades the target mRNA and is a molecule that plays a negative regulatory role. Current studies have shown that many miRNAs are involved in physiological processes such as organism development, differentiation, growth, and immune response, and their expression and dysfunction may lead to various pathol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/113
Inventor 何祥火顾健人杨胜利万大方李文晰
Owner SHANGHAI INST OF ONCOLOGY
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