Method for evaluating united action in acute and toxic alga infrared test

Abstract

The invention relates to a method for evaluating a united action in an acute and toxic alga infrared test. The method is characterized in that a united action coefficient theta and a total average deflection coefficient f are used for constructing a united deflection coefficient method (the united deflection coefficient is represented by using theta i), and the f equal to 0.06 is used for adjusting the theta theoretical value of an additive action (the theta is equal to 1). Evaluating according to the change of the united deflection coefficient theta i, the evaluating method is as follows: the theta i is greater than 1 plus the f, namely, the theta i greater than 1.06 is a synergistic action; the theta i is greater than or equal to 1 minus the f and is smaller than or equal to 1 plus the f, namely, the theta i greater than or equal to 0.94 and smaller than or equal to 1.06 is the additive action; the theta i is smaller than 1 minus the f, namely, the theta i smaller than 0.94 is an antagonistic action; and T beta greater than T phi and smaller than T lambada minus the f*the T lambada is an independent action (the T phi is a temperature difference generated by a medicine with stronger toxic effect, the T beta is a temperature difference of the united function, the T lambada is a temperature difference of the additive action, and the f*the T lambada is deflection temperature difference). In the invention, the test method is simple and convenient; a binary medicine represents four kinds of united action types comprising the synergistic action, the additive action, the antagonistic action and the independent action to a sensitive alga action; and the use time of the alga infrared test method for completing a test experiment for one time is less than 60 minutes and is far lower than the period of 24 hours to 96 hours of an experiment using the light density, the cell number, the chlorophyll content and the dry cell weight as test indexes to carry out a toxicity test.

Description

technical field [0001] The invention relates to an evaluation method for the joint action in the infrared test of acute toxicity algae, and belongs to the technical field of ecotoxicology. Background technique [0002] The comprehensive biological effect produced by the joint action of two or more chemical pollutants is called joint action. In the environment, multiple pollutants often exist at the same time, and the combined effects of multiple and single chemical pollutants on organisms are completely different. Therefore, one of the research trends in ecotoxicology is to strengthen the comprehensive effects of pollution and the environmental effects of multi-component composite pollution. (Kong Fanxiang. 2000. "Environmental Biology" [M]. Beijing: Higher Education Press: 13, 92-93). However, the study of these two effects requires the evaluation method of joint action and the establishment of the type of joint action of toxicants, there is no standard method (Kong Fanxia...

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Problems solved by technology

At present, the acute toxicity of heavy metals in the environment has been tested by algae infrared radiation (Guo Weihua, Su Haiyan, Zhang Zhi, Wang Bin, You Xiantong. 2008a. The acute toxicity of heavy metals in the environment by algae infrared radiation test [J]. Ecological Environment, 17(2): 520- 523), Acute Toxicity of Pesticide Residues in Algae Infrared Test (Guo Weihua, Wang Xiang, Zhang Zhi, You Xiantong, Wang Bin. 2008b. Study on Acute Toxicity of Pesticide Residues in Algae Infrared Test Environment [J]. Journal of Agricultural Environmental Science, 27(5): 2039-2042), the determination method of sensitive algae in the infrared test of acute toxic algae (Guo Weihua, Ding Yanyan, Zhang Zhi, Wang Xiang, Su Haiyan. 2008c. The determination method of sensitive algae in the infrared test of acute toxic algae[J]. Ecotoxicology Chinese Journal, 3(6): 577-583), however, there is no report on the joint effect of algae infrared test method at home and abroad.

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