Development of capillary electrophoresis-chemiluminescence detecting device

A technology of chemiluminescence detection and capillary electrophoresis, which is used in chemiluminescence/bioluminescence, measurement devices, and analysis by chemically reacting materials, which can solve the problems of limited application and poor selectivity.

Inactive Publication Date: 2009-12-30
郗娟 +2
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  • Abstract
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  • Application Information

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Problems solved by technology

However, this method has poor selectivity, which limits its application in practical analysis to a certain extent.

Method used

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  • Development of capillary electrophoresis-chemiluminescence detecting device
  • Development of capillary electrophoresis-chemiluminescence detecting device

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Embodiment Construction

[0013] The capillary electrophoresis-chemiluminescence detection device developed by the present invention mainly has the technical points of the following two aspects:

[0014] 1. The self-processed PTFE four-way interface is used to fix the capillary, and the ground electrode is inserted into the fourth port. The interface method is the post-column casing type. The screw port of the electrode interface should be sealed as much as possible to reduce the influence of diffusion on the reproducibility. The waste liquid after the column is led into the waste liquid cup by a glass rod. This interface mode saves the electrophoresis buffer solution and brings great convenience to the operation.

[0015] 2. Use a micro syringe pump to push the post-column chemiluminescence reagent into the interface. The syringe pump can deliver trace solutions continuously and without pulse, which brings great convenience to the operation. The flow rate of the luminescence reagent is a very impor...

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Abstract

The invention belongs to the development field of novel analytical instruments, which relates to works with respect to designing, constructing and debugging of a coupled instrument of capillary electrophoresis separation and chemiluminescence method detection. The capillary electrophoresis-chemiluminescence detecting device developed by the invention mainly has the following two aspects of technical essentials: (1) a self-processed polytetrafluoroethylene four-way connector is used for fixing the capillary, and the connector is in a post-column sleeve type; (2) a post-column chemiluminescence reagent is pushed into the connector by using a micro-injection pump. The capillary electrophoresis-chemiluminescence detecting device has convenient operation and good reproducibility and is debugged by a tris-(2, 2'-bipyridine) ruthenium (II) [Ru (bipy)3<2+>]-H2C2O4-Ce (IV) chemiluminescence system. Under the experiment condition of preliminary optimization, capillary electrophoresis separation and chemiluminescence detection of H2C2O4 are realized. The result shows that the detecting device developed by the invention can basically meet the requirements of scientific research.

Description

technical field [0001] The invention belongs to the field of research and development of new analytical instruments, and relates to the design, construction and debugging of a combined instrument for capillary electrophoresis separation and chemiluminescence detection. Background technique [0002] Capillary electrophoresis is a new high-efficiency separation technology that came out in the 1980s. It is the product of the combination of classic electrophoresis technology and modern micro-column separation. Its appearance has received great attention, and it has become one of the most active research directions in analytical chemistry, and is widely used in the separation and analysis of proteins, drugs, DNA, and environmental pollutants. As far as capillary electrophoresis is concerned, the extremely fine inner diameter of the capillary brings high separation efficiency, but also brings difficulties to the detection of sample components. How to increase the sensitivity of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N21/76
Inventor 郗娟樊丙安魏春龙
Owner 郗娟
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