Test paper for detecting deer epizootic haemorrhagic disease virus and preparation method thereof

A deer epidemic hemorrhage and virus detection technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problem of low sensitivity and achieve high sensitivity, strong specificity, and rapid detection

Inactive Publication Date: 2010-01-13
花群义
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a test strip for detection of deer epidemic hemorrhagic disease virus and a preparation method for the above-mentioned problem of low sensitivity of the existing deer epidemic hemorrhagic disease quarantine method

Method used

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  • Test paper for detecting deer epizootic haemorrhagic disease virus and preparation method thereof

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Experimental program
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Embodiment Construction

[0027] Such as figure 1 Shown is a schematic diagram of an embodiment of the test strip for detection of deer epidemic hemorrhagic disease virus of the present invention. In this embodiment, the test strip includes a base plate 7 and a sample absorbent pad 1, a nitrocellulose membrane 3 and a water absorbent pad 6 fixed on the base plate 7 in sequence, wherein the sample absorbent pad 1 includes a colloidal gold pad 2, and the colloidal gold Pad 2 is a porous fiber adsorbed with colloidal gold-labeled monoclonal antibody. The nitrocellulose membrane 3 is provided with a detection line 4 and a control line 5 parallel to each other. The detection line 4 is coated or sprayed with polyclonal deer epidemic hemorrhagic disease virus. Antibody, control line 5 coated or sprayed with recombinant protein A (protein A).

[0028] In the above test strip, the porous fibers may be glass fibers or other materials with similar structures. The detection line 4 and the detection line 5 are pa...

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Abstract

The invention relates to a test paper for detecting deer epizootic haemorrhagic disease virus, comprising a soleplate (7), and a sample absorption pad (1), a nitrocellulose membrane (3) and a water absorption pad (6) which are sequentially fixed on the soleplate, wherein the sample absorption pad comprises a colloid metal pad (2) which is porous fiber absorbed with colloid metal labeling of monoclonal antibodies, the nitrocellulose membrane is provided with parallel detection lines (4) and comparison lines (5), and the detection lines are coated or sprayed with deer epizootic haemorrhagic disease virus monoclonal antibodies, and the comparison lines are coated or sprayed with recombination protein. The invention also provides a preparation method of the test paper which can directly and rapidly detect the deer epizootic haemorrhagic disease virus monoclonal antigen, and has strong idiosyncracy, high sensitivity and rapid detection.

Description

technical field [0001] The invention relates to the field of virus detection, in particular to a rapid detection test strip for detecting deer epidemic hemorrhagic disease virus antigen, and a preparation method of the rapid detection test strip. Background technique [0002] Epizootic haemorrhagic disease of deer (EHD) is a non-contact viral infectious disease caused by the deer epidemic haemorrhagic disease virus (EHD) of the family Reoviridae. (Epizootic hemorrhagic disease virus of deer, EHDV) and spread by arthropods (mainly Culicoides). Livestock such as cattle and sheep and various domesticated and wild ruminants such as white-tailed deer, moose, and eland antelope can be infected with the disease. The genome of EHDV is similar to that of BTV, and eight serotypes have been found so far. [0003] Deer epidemic hemorrhagic disease first occurred in the United States, and the fatality rate was as high as 62%. In 1960, the disease was prevalent in Japanese cattle herds...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 花群义杨俊兴阮周曦陈兵曾少灵曹琛福秦智锋吕建强陶虹林庆燕
Owner 花群义
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