Method for modifying DNA microfluidic chip micro-channel with mixed screening medium

A microfluidic chip and microchannel technology, applied in stress-stimulated microbial growth methods, DNA preparation, recombinant DNA technology, etc., can solve problems such as poor analysis reliability, long time for DNA analysis of biochemical samples, and short service life of modifiers , to achieve the effect of long service life, simple and easy operation steps and good screening effect

Inactive Publication Date: 2010-02-24
EAST CHINA NORMAL UNIVERSITY
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Problems solved by technology

In the background technology, there is a method for modifying the microchannel of a DNA microfluidic chip with a single sieving medium, using a single sieving medium as a modifier, and when the microchannel is modified by this method for the analysis

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  • Method for modifying DNA microfluidic chip micro-channel with mixed screening medium
  • Method for modifying DNA microfluidic chip micro-channel with mixed screening medium
  • Method for modifying DNA microfluidic chip micro-channel with mixed screening medium

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[0030] Example

[0031] This embodiment completely follows the specific operation steps of the above-mentioned method and is concise. The following only lists the key technical data of each step.

[0032] In the first step, the added amount of polyvinylpyrrolidone and hydroxyethyl cellulose are both 6.8 mg, and the percentage concentration of the modifier is 2.5%; in the third step, the fluorescent dye is a fluorescent cyanine monomer dye ; In the fourth step, the capacity of the medical syringe is 1 mL.

[0033] The electrophoresis process of the modified DNA microfluidic chip of this embodiment for DNA sample analysis:

[0034] In this example, a 0.5 μg / μL ΦX 174-HaeIII digest DNA marker was taken and diluted 10-fold with ultrapure water as a DNA sample.

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Abstract

A method for modifying a DNA microfluidic chip micro-channel with a mixed screening medium belongs to the technical field of DNA microfluidic chip electrophoretic analysis. and includes following steps: preparing a mixed screening medium of polyvinylpyrrolidone and hydroxyethyl cellulose as a modifier and modifying the DNA microfluidic chip micro-channel with the modifier. The method has easy operation. When the DNA microfluidic chip with the modified micro-channel is analyzed of DNA segments, the biochemical sample DNA screening effect is good; the analysis time is short and modifier servicelife is long. The invention is especially adapted to modifying the micro-channel of the DNA microfluidic chip to make the modified chip become the qualified and efficient DNA electrophoretic analysisimplement.

Description

technical field [0001] The invention relates to a method for modifying the microchannel of a DNA microfluidic chip with a mixed sieving medium, belonging to the technical field of DNA microfluidic chip electrophoresis analysis. Background technique [0002] With the rise of microfluidic chip analysis technology, DNA microfluidic chips are increasingly used in life sciences, and are increasingly used in the separation and sequencing of DNA fragments. DNA microfluidic chip analysis has the characteristics of fast analysis speed, low reagent consumption, and reusable chip. However, the adsorption of biochemical samples by the microchannel of the chip limits the general promotion of this technology to a certain extent. Therefore, on the basis of ensuring the reliability and repeatability of chip analysis, modifying the microchannels of DNA microfluidic chips has become one of the keys to popularize DNA microfluidic chip analysis technology and industrialization. In the backgrou...

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Application Information

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IPC IPC(8): C12M1/42C12N15/10C12Q1/68G01N21/64
Inventor 李钢葛淑丽王清江何品刚方禹之
Owner EAST CHINA NORMAL UNIVERSITY
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