Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for modifying DNA microfluidic chip micro-channel with mixed screening medium

A microfluidic chip and microchannel technology, applied in stress-stimulated microbial growth methods, DNA preparation, recombinant DNA technology, etc., can solve problems such as poor analysis reliability, long time for DNA analysis of biochemical samples, and short service life of modifiers , to achieve the effect of long service life, simple and easy operation steps and good screening effect

Inactive Publication Date: 2010-02-24
EAST CHINA NORMAL UNIVERSITY
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the background technology, there is a method for modifying the microchannel of a DNA microfluidic chip with a single sieving medium, using a single sieving medium as a modifier, and when the microchannel is modified by this method for the analysis of DNA fragments, the microfluidic The adsorption of biochemical samples by the channel is greatly reduced, but the analysis time for biochemical sample DNA is longer, the analysis reliability is poor, and the service life of the modifier is shorter

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for modifying DNA microfluidic chip micro-channel with mixed screening medium
  • Method for modifying DNA microfluidic chip micro-channel with mixed screening medium
  • Method for modifying DNA microfluidic chip micro-channel with mixed screening medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] This embodiment operates completely according to the specific operation steps of the above-mentioned method. For the sake of brevity, only key technical data of each step are listed below.

[0032] In the first step, the addition amount of polyvinylpyrrolidone and hydroxyethyl cellulose is 6.8 mg, and the percentage concentration of the modification agent obtained is 2.5%; in the third step, the fluorescent dye is a fluorescent cyanine monomer dye ; In the fourth step, the volume of the medical syringe is 1mL.

[0033] The electrophoresis process of DNA sample analysis by DNA microfluidic chip modified in this example:

[0034] In this example, 0.5 μg / μL of ΦX 174-HaeIII digest DNA marker was taken and diluted 10 times with ultrapure water as a DNA sample.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method for modifying a DNA microfluidic chip micro-channel with a mixed screening medium belongs to the technical field of DNA microfluidic chip electrophoretic analysis. and includes following steps: preparing a mixed screening medium of polyvinylpyrrolidone and hydroxyethyl cellulose as a modifier and modifying the DNA microfluidic chip micro-channel with the modifier. The method has easy operation. When the DNA microfluidic chip with the modified micro-channel is analyzed of DNA segments, the biochemical sample DNA screening effect is good; the analysis time is short and modifier servicelife is long. The invention is especially adapted to modifying the micro-channel of the DNA microfluidic chip to make the modified chip become the qualified and efficient DNA electrophoretic analysisimplement.

Description

technical field [0001] The invention relates to a method for modifying the microchannel of a DNA microfluidic chip with a mixed sieving medium, belonging to the technical field of DNA microfluidic chip electrophoresis analysis. Background technique [0002] With the rise of microfluidic chip analysis technology, DNA microfluidic chips are increasingly used in life sciences, and are increasingly used in the separation and sequencing of DNA fragments. DNA microfluidic chip analysis has the characteristics of fast analysis speed, low reagent consumption, and reusable chip. However, the adsorption of biochemical samples by the microchannel of the chip limits the general promotion of this technology to a certain extent. Therefore, on the basis of ensuring the reliability and repeatability of chip analysis, modifying the microchannels of DNA microfluidic chips has become one of the keys to popularize DNA microfluidic chip analysis technology and industrialization. In the backgrou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/42C12N15/10C12Q1/68G01N21/64
Inventor 李钢葛淑丽王清江何品刚方禹之
Owner EAST CHINA NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com