Simple and convenient establishment method of virus in-vitro three-dimensional culture model

Abstract

The invention provides a simple and convenient establishment method of a virus in-vitro three-dimensional culture model. The establishment method comprises the following operating steps: (1) preparing a micro-carrier suspension; (2) preparing a cell adherent retardant; and (3) preparing a six-pore plate; uniformly paving the prepared cell adherent retardant in the six-pore plate; keeping and air-drying the six-pore plate; then, inoculating the six-pore plate with prepared cells by a concentration of 10<5> / ml, and adding the prepared micro-carrier suspension, wherein the ratio of cell liquid to the micro-carrier suspension is at 4: 1; uniformly mixing the cell liquid and the micro-carrier suspension; keeping an obtained mixture in a cell incubator; and implementing a virus inoculation experiment as a cell three-dimensional structure is formed after 3-4 days. According to the method, high cost caused by an animal in-vivo experiment can be avoided, and meanwhile, an animal in-vivo growth model can be simulated to the greatest extent, so that the cultivated cells follow a three-dimensional growth mode, and a similar in-vivo tissue structure is formed; therefore, the establishment method is beneficial for simulating a virus in-vivo pathogenesis process; and the model establishment method is simple, low in cost and convenient for popularization.

Description

technical field [0001] The invention relates to an in vitro model for virus culture, in particular to a simple method for establishing a three-dimensional virus culture model in vitro. Background technique [0002] In the development history of virology, susceptible animals such as rabbits, mice, and guinea pigs were often used to conduct experiments on the target virus. Animal models can be used to observe the invasion of the virus on the body and the site of pathological changes, but individual animals vary greatly, are expensive, and require special animal rooms, so the cost of promotion is huge. With the development of science, many models of in vitro cultured viruses have emerged, but conventional cell culture technology models often cannot reflect the pathogenic process of viruses in vivo. Contents of the invention [0003] Purpose of the invention: In order to overcome the above deficiencies, the present invention provides a simple method for establishing a three-d...

AI Technical Summary

Problems solved by technology

Animal models can observe the damage of the virus to the body and the parts that cause pathological changes, but individual animals vary greatly, are expensive, and require special animal rooms, so the cost of promotion is huge

With the development of science, many models of in vitro cultured viruses have emerged, but conventional cell culture technology models often cannot reflect the pathogenic process of viruses in vivo

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