Method for constructing dendrobium officinale polysaccharide fingerprinting

The technology of Dendrobium officinale polysaccharide and fingerprint spectrum is applied in the directions of pharmaceutical formulations, preparation of test samples, and medical preparations containing active ingredients, which can solve the problems of high cost and complicated operation, and achieve the effect of simple operation and high efficiency.

Inactive Publication Date: 2010-06-02
HEFEI UNIV OF TECH
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  • Abstract
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Problems solved by technology

[0004] In order to solve the problem of complex identification operation and high cost in the existing Dendrobium officinale, the present invention provides a method for constructing the polysaccharide fingerprint of Dendrobium officinale by using fluorescence-assisted glycophoresis technology

Method used

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  • Method for constructing dendrobium officinale polysaccharide fingerprinting
  • Method for constructing dendrobium officinale polysaccharide fingerprinting
  • Method for constructing dendrobium officinale polysaccharide fingerprinting

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Embodiment

[0056] Taking Dendrobium officinale in Wenzhou, Zhejiang as an example, the specific operation steps are as follows:

[0057] (1) Extraction of polysaccharides from Dendrobium officinale in Wenzhou, Zhejiang:

[0058] Take 10.0 g of dried stem powder of Dendrobium officinale from Wenzhou, Zhejiang, add 100 mL of double distilled water, and extract in a water bath at 70 ° C for 4 h, repeating 3 times. The extract was collected, concentrated under reduced pressure to 50 mL, added with 200 mL of 95% ethanol (analytical grade), allowed to stand at 4°C for 24 h, and centrifuged (15 min, 12000×g) to collect the precipitate. The collected precipitate was evaporated to ethanol, dissolved with 10 mL of double distilled water, and deproteinized with 5 mL of protein removal reagent (chloroform: n-butanol = 4: 1, v / v), centrifuged to collect the supernatant, and freeze-dried to obtain 1 g Zhejiang Wenzhou iron sheet Dendrobium polysaccharides.

[0059] (2) Enzymatic hydrolysis of polysa...

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Abstract

The invention relates to a method for constructing dendrobium officinale polysaccharide fingerprinting, which concretely comprises the following seven operation steps: extracting dendrobium officinale polysaccharide, carrying out zymohydrolysis on the dendrobium officinale polysaccharide, deriving a dendrobium officinale polysaccharide zymolyte, constructing an oligosaccharide scale, deriving oligosaccharide of different polymerization degrees and constructing the dendrobium polysaccharide fingerprinting of different producing areas and different varieties. The method of extracting the dendrobium officinale polysaccharide of different producing areas and different varieties, the method of carrying out zymohydrolysis on the polysaccharide and the method of deriving a polysaccharide zymohydrolysis product are the same as the steps (1), (2) and (3) and can be respectively operated simultaneously. The method for constructing the dendrobium officinale polysaccharide fingerprinting by utilizing a fluorescent auxiliary sugar electrophoresis technology has the advantages of simple and sensitive operation, high efficiency and the like, is a novel method for identifying and evaluating traditional Chinese medicinal materials and makes up for the defect that the traditional method for identifying and evaluating the traditional Chinese medicinal materials is lack of the relevant information of medicinal active substances.

Description

technical field [0001] The invention belongs to the field of Chinese herbal medicine resources, and in particular relates to a method for constructing a dendrobium officinale polysaccharide fingerprint. Background technique [0002] Dendrobium officinale is one of the precious medicinal plants clearly recorded in the Chinese Pharmacopoeia and herbal medicines of past dynasties, and polysaccharides are the main material basis for its pharmacological effects. Modern pharmacological studies have shown that Dendrobium officinale has various biological effects such as anti-fatigue, anti-oxidation, enhancing immunity, anti-tumor, anti-platelet aggregation, and lowering blood sugar. In recent years, Dendrobium candidum has been developed into a variety of health functional foods, such as Dendrobium candidum, which is expensive. Due to the special requirements for the growth environment of Dendrobium officinale, low self-reproductive coefficient and over-harvesting by people, its w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8984A61P39/00A61P39/06A61P37/04A61P35/00A61P7/02A61P3/10G01N27/447G01N1/28A61K135/00
Inventor 罗建平查学强王军辉潘利华魏鹏
Owner HEFEI UNIV OF TECH
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