Method for enhancing root inducing of Angustmycin in production of Grosvener Siraitia tissue culture plantlet

The technology of Lingfasu and Luohanguo is applied in the field of strengthening the root-promoting effect of Lingfasu in the production of Luohanguo tissue culture seedlings, which can solve the problems such as threatening the genetic stability of the tissue culture seedlings, low resistance, etc. The adventitious root system is developed and the effect of strengthening the root-promoting effect

Inactive Publication Date: 2010-06-16
GUANGXI UNIV
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Problems solved by technology

Moreover, BA will inevitably induce a large amount of callus in the tissue culture of Luo Han Guo, and with the whole process of production, it directly threatens the genetic stability of the tissue culture seedlings, leading to various inferior variations and adverse events (such as low resistance) in the field cultivation. , not normal flowering and fruiting, etc.), brings great potential risks to producers and growers of tissue culture seedlings

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  • Method for enhancing root inducing of Angustmycin in production of Grosvener Siraitia tissue culture plantlet
  • Method for enhancing root inducing of Angustmycin in production of Grosvener Siraitia tissue culture plantlet

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Embodiment Construction

[0009] According to the fact that both lingfasu and arginine have the biological activity of promoting cell division and adventitious roots, the applicant has repeatedly screened and studied the use concentration and ratio, using MS solid medium as the basic medium, adding 0.2mg of lingfasu L -1 And arginine 0.5mg·L -1 , to make an improved MS solid medium for the production of Luo Han Guo tissue culture seedlings. The aseptic culture of the single-segment stem with axillary buds of Luo Han Guo can be directly obtained in the two technical links of "explant initiation" and "subculture multiplication" with complete roots and seedlings, less callus, and special root systems. Well-developed regenerated plants can be transplanted directly out of the bottle, and can also continue to proliferate, while completely omitting the "rooting" link in traditional production techniques.

[0010] The morphological effect (cultivation 25 days) of arginine strengthens the rooting effect of Li...

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Abstract

The invention provides a method for enhancing the root inducing of Angustmycin in production of Grosvener Siraitia tissue culture plantlet. In the production process, MS solid medium is utilized in the production process as the basic medium, and the Grosvener Siraitia single link stems with buds are taken as explants. The method is characterized in that Angustmycin and spermine are added into the MS solid medium, and regenerated plants with complete roots and seedlings, few callus and developed and robust root system can be obtained in the two production processes of starting cultivating of explants and subculture enrichment culture, which can be transplanted out of bottles and can also be propagated and enlarged continuously. Conventional cultivation needs 25 days, the number of roots of each plant is great than or equal to 8, and the process of generating roots in the traditional method can be completely omitted.

Description

technical field [0001] The invention belongs to a method for strengthening the root-promoting effect of lingfasu in the production of Luo Han Guo tissue culture seedlings. technical background [0002] Luo Han Guo Siraitia grosvenori (Swingle) C. Jeffrey, also known as Han Guo, Lahan Guo, Longevity Fruit, Fairy Fruit, Luo Huangzi, False Bitter Melon, Light Fruit Wood Softshell Turtle, etc., is a perennial herbaceous vine of the genus Siraitia Merr of Cucurbitaceae Cucurbitaceae, male and female Due to the high application value and broad development prospects in medical and health care, heterogeneous plants have been continuously developed in planting scale, and tissue culture seedlings have occupied an absolute dominant position in the provenance of Luo Han Guo. However, most of the production units of Luo Han Guo tissue culture seedlings use BA (N 6 -benzyl adenine) and growth regulators such as IAA (indole acetic acid), NAA (naphthalene acetic acid) or IBA (indole butyri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 许鸿源
Owner GUANGXI UNIV
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