Method for enhancing root inducing of Angustmycin in production of Grosvener Siraitia tissue culture plantlet

Abstract

The invention provides a method for enhancing the root inducing of Angustmycin in production of Grosvener Siraitia tissue culture plantlet. In the production process, MS solid medium is utilized in the production process as the basic medium, and the Grosvener Siraitia single link stems with buds are taken as explants. The method is characterized in that Angustmycin and spermine are added into the MS solid medium, and regenerated plants with complete roots and seedlings, few callus and developed and robust root system can be obtained in the two production processes of starting cultivating of explants and subculture enrichment culture, which can be transplanted out of bottles and can also be propagated and enlarged continuously. Conventional cultivation needs 25 days, the number of roots of each plant is great than or equal to 8, and the process of generating roots in the traditional method can be completely omitted.

Description

technical field [0001] The invention belongs to a method for strengthening the root-promoting effect of lingfasu in the production of Luo Han Guo tissue culture seedlings. technical background [0002] Luo Han Guo Siraitia grosvenori (Swingle) C. Jeffrey, also known as Han Guo, Lahan Guo, Longevity Fruit, Fairy Fruit, Luo Huangzi, False Bitter Melon, Light Fruit Wood Softshell Turtle, etc., is a perennial herbaceous vine of the genus Siraitia Merr of Cucurbitaceae Cucurbitaceae, male and female Due to the high application value and broad development prospects in medical and health care, heterogeneous plants have been continuously developed in planting scale, and tissue culture seedlings have occupied an absolute dominant position in the provenance of Luo Han Guo. However, most of the production units of Luo Han Guo tissue culture seedlings use BA (N 6 -benzyl adenine) and growth regulators such as IAA (indole acetic acid), NAA (naphthalene acetic acid) or IBA (indole butyri...

AI Technical Summary

Problems solved by technology

Moreover, BA will inevitably induce a large amount of callus in the tissue culture of Luo Han Guo, and with the whole process of production, it directly threatens the genetic stability of the tissue culture seedlings, leading to various inferior variations and adverse events (such as low resistance) in the field cultivation. , not normal flowering and fruiting, etc.), brings great potential risks to producers and growers of tissue culture seedlings

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