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Homocysteine diagnosis/determination reagent (kit) and homocysteine concentration determination method

A homocysteine ​​and reagent technology, applied in the field of medical testing and determination, can solve the problems of complicated operation, long test time, large test data variation, etc.

Inactive Publication Date: 2010-06-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but it has the disadvantages of complicated operation, long test time and large variation of test data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0049] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0050] Stabilizer 500mmol / L

[0051] Coenzyme 3mmol / L

[0052] Thiatine-homocysteine ​​methyltransferase 10000U / L

[0053] Methionine adenosyltransferase 12000U / L

[0054] Glyceraldehyde-3-phosphate dehydrogenase 12000U / L

[0055] Dimethylsulfonium acetate 7mmol / L

[0056] Adenosine triphosphate 5mmol / L

[0057] Glyceraldehyde-3-phosphate 9mmol / L

[0058] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0059] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, volume ratio of tested homocysteine ​​sample to reagent is 1 / 25, the reaction d...

Embodiment 2

[0062] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a dual reagent, including:

[0063] Reagent 1

[0064] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0065] Stabilizer 50mmol / L

[0066] Coenzyme 3mmol / L

[0067] Dimethylsulfonium acetate 7mmol / L

[0068] Adenosine triphosphate 5mmol / L

[0069] Glyceraldehyde-3-phosphate 9mmol / L

[0070] Reagent 2

[0071] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0072] Stabilizer 50mmol / L

[0073] Thiatine-homocysteine ​​methyltransferase 10000U / L

[0074] Methionine adenosyltransferase 12000U / L

[0075] Glyceraldehyde-3-phosphate dehydrogenase 12000U / L

[0076] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0077] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test s...

Embodiment 3

[0080] The homocysteine ​​diagnosis / measurement reagents of this embodiment are three reagents, including:

[0081] Reagent 1

[0082] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0083] Stabilizer 50mmol / L

[0084] Coenzyme 3mmol / L

[0085] Dimethylsulfonium acetate 7mmol / L

[0086] Adenosine triphosphate 5mmol / L

[0087] Glyceraldehyde-3-phosphate 9mmol / L

[0088] Reagent 2

[0089] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0090] Stabilizer 500mmol / L

[0091] Methionine adenosyltransferase 12000U / L

[0092] Glyceraldehyde-3-phosphate dehydrogenase 12000U / L

[0093] Reagent 3

[0094] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0095] Stabilizer 500mmol / L

[0096] Thiatine-homocysteine ​​methyltransferase 10000U / L

[0097] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0098] When measuring th...

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Abstract

The invention relates to a homocysteine diagnosis / determination reagent (kit) using enzyme-colorimetry and enzyme-linked method technology. Meanwhile, the invention also relates to a homocysteine concentration determination method, reagent compositions and components, which belong to the technical filed of medical test and determination. The reagent (kit) of the invention mainly includes: buffer solution, coenzyme, dimethyl sulfenyl acetate, adenosine triphosphoric acid, glyceraldehyde-3-phosphate, thetin-homocysteine methyltransgerase, methionine adenylyltransferase, glyceraldehyde-3-phosphate dehydrogenase and stabilizing agent; samples and reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions; then the reactants are arranged under an ultraviolet / visible light analyzer to detect the increasing degree of absorbance at the 340nm position of a dominant wave so as to measure and calculate the concentration of the homocysteine.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnosis / measurement reagent (box), and at the same time, the invention also relates to a method for measuring the homocysteine ​​concentration, which belongs to the technical field of medical examination and measurement. Background technique [0002] There are many methods for measuring homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, fluorescence Polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] 1. High-performance liquid chromatography (HPLC): It is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. In clinical application, it is gradually adopted by enzyme-linked immunoassay, Fluorescence polarization analysis and enzymatic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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