Method for measuring content of IgY in yolk and/or serum

Abstract

The invention discloses a method for measuring the content of IgY in yolk and/or serum. The method comprises the following steps: 1) coating an ELISA (enzyme-linked immuno sorbent assay) plate with an IgY antibody; 2) adding yolk or serum IgY antibody solution to the ELISA plate in step 1); 3) adding an enzyme-labeled antibody resisting the IgY antibody to the ELISA plate in step 2) and carrying out measuring by an enzyme-labeling instrument. The method in the invention has the following advantages: (1) the operating steps are simple, and the early-stage preparation only comprises blood collection or crude extraction of IgY; (2) as more samples can be measured at the same time, the efficiency is high; (3) the sensitivity is high. When only the content of IgY is needed for analysis, the method in the invention can quickly and sensitively obtain the measuring result, thereby saving time, cost and manpower; when a final purified product is needed, the method in the invention can be used as a content parameter to not only predict the content of IgY in the purified product but also provide comparison to indicate the purifying efficiency.

Description

technical field [0001] The invention relates to a method for measuring IgY content in egg yolk and / or serum, which is especially suitable for the fields of immune detection, poultry breeding and the like. Background technique [0002] IgY is ubiquitous in birds, reptiles, and amphibians and is functionally equivalent to mammalian IgG, but many of its biological properties remain undiscovered. In recent years, many researchers have analyzed the gene composition and biological function of IgY, confirming that it is the direct origin of mammalian IgG and IgE. IgY has high added value. The main physiological functions in the body are agglutination, phagocytosis, and neutralization of bacteria and toxins, enhancing the body's ability to prevent and resist diseases. It is the modern food industry that caters to the physiological needs of special groups of people (especially infants and Food for the elderly) is an ideal immune enhancer. [0003] At present, the IgY antibody in eg...

AI Technical Summary

Problems solved by technology

[0003] At present, the IgY antibody in egg yolk is generally extracted by water dilution method, and then graded precipitation is carried out with organic solvents such as ethanol, or various methods such as sulfate precipitation, ion exchange chromatography, gel filtration or ultrafiltration are used to realize IgY antibody. Although the products obtained by these methods have high purity, most of these methods have disadvantages such as many operation steps, small batch size, large reagent consumption and recovery rate, and the IgY obtained after various purification steps reflects its presence in The content in egg yolk is not accurate enough. If you only need to use egg yolk or serum IgY content for analysis, and do not need purified products, this method is too time-consuming and laborious, especially when a large amount of data is required for analysis, its disadvantages are more obvious

Images