Uranium-related gene segments of pokeweed and acquiring method and application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of gene fragments and pokeweed, applied in the fields of molecular biology and environmental monitoring
Inactive Publication Date: 2010-08-04
HUNAN UNIV OF SCI & TECH
View PDF0 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
But so far, this method has not been reported in China
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0032] 1. Take the pokeweed root tissue from the uranium tailings area and the non-polluted control area, and store it in liquid nitrogen at -196°C until use;
[0033] 2. Extract total RNA from pokeweed root tissue, such as figure 1
[0034] (1) Get pre-cooled RNA extraction buffer (4mol / L guanidine isothiocyanate, 25mmol / L sodium citrate (pH7.0), 0.5% (W / V) sodium lauryl sarcosine, 4 % (V / V) β-mercaptoethanol (added before use)) 10mL was added to a 50mL centrifuge tube without RNase, and placed on ice;
[0035] (2) Weigh 4g of pokeweed root system and grind it fully in a mortar with excess liquid nitrogen, transfer the pokeweed root system powder into a centrifuge tube with a pre-cooled medicine spoon, shake vigorously for 10 minutes, and stand on ice for 5 minutes ;
[0036] (3) Add 1mL 2mol / L sodium acetate (pH 4.0) and shake well;
[0037] (4) Add 10 mL of water-saturated phenol (pH 4.5) and shake well;
[0038] (5) Add 2 mL of chloroform:isoamyl alcohol (24:1), shake...
Embodiment 2
[0155] 1. The steps are the same as in Example 1;
[0156] 2. The steps are the same as in Embodiment 1 and 2;
[0157] 3. Step is the same as embodiment one 3;
[0158] 4. Step is the same as embodiment one 4;
[0159] 5. The steps are the same as in Embodiment 1 and 5;
[0160] 6. The steps are the same as in Example 1; 6;
[0161] 7. Design primers based on the sequences at both ends of the specifically expressed gene fragment;
[0162] 8. Do RT-PCR to detect pokeweed cDNA in the environment.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses uranium-related gene segments of pokeweed. The method for acquiring the gene segments comprises the following steps: extracting total RNA from root tissues of pokeweed in uranium tailings region and non-polluted contrast region, synthesizing a first chain and a second chain of cDNA by enzymatic reaction reverse transcription, carrying out suppression subtractive hybridization to separate gene segments with specific expression under heavy metal uranium stress, using the gene segments as probes and enabling the gene segments and RNA extracted from pokeweed in detected environment to hybridize, or using sequences of two ends of the gene segments with specific expression as primers to carry out reverse transcription-polymerase chain reaction to detect the cDNA of pokeweed in the detected environment. In the invention, gene with specific expression induced by uranium in pokeweed is used as a probe to detect uranium pollution, solving the problem of inaccurate detection of uranium pollution in surrounding environment of uranium-polluted areas.
Description
technical field [0001] The present invention belongs to the fields of molecular biology and environmental monitoring, and specifically discloses a gene fragment related to uranium in pokeweed and a method for obtaining the gene fragment, and further relates to the use of the gene fragment in detecting uranium pollution in the environment application. Background technique [0002] The process of uranium mining and nuclear energy utilization is an important way for uranium to be enriched and polluted in the environment. Uranium tailings and waste rock contain uranium and all its daughters, and its radionuclide content is 2 to 3 orders of magnitude higher than the background. Improper accumulation or disposal of these wastes can result in uranium being dispersed to the soil surface through runoff, into the air through wind erosion, or into groundwater through leaching. In addition, the uranium ore smelting process will continuously discharge a large amount of radioactive wast...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more