Rapid in-vitro propagation method and application of anemone flaccida fr.schmidt

Abstract

The invention belongs to the technical field of plant tissue culture, in particular relating to a rapid in-vitro propagation method and application of anemone flaccida fr.schmid. The invention is characterized by optimizing the key technical problems of adventitious bud propagation, protocol corm induction, rhizogenesis culture, seedling hardening transplantation and the like of in-vitro anemone flaccida fr.schmidt by screening culture medium and culture condition. In the invention, the multiplication coefficient is 4.11, the seedling propagation coefficient is not less than 3.0, the induction effect on protocol corm is significant and the induction ratio is 76.5%. The test tube seedling of the anemone flaccida fr.schmid cultured by the invention has the rhizogenesis ratio of 97.2%, and the regeneration root is robust. By applying the seedling hardening transplantation method of the invention, the rate of germination of the season is higher than 90%, and the transplantation survival rate is at least 95%.

Description

technical field The invention belongs to the technical field of plant tissue culture, and in particular relates to an in vitro rapid multiplication culture method and application of tree shade anemones. technical background Anemone flaccida Fr. Schmidt is a perennial herb of the genus Anemone in the family Ranunculacease. Its dry rhizome is commonly known as "Diwu", which has the functions of dispelling rheumatism, strengthening muscles and bones, reducing swelling and relieving pain. Rare alpine herbs. From March to May every year is the growth and development period of the aboveground part of the shade anemone. Basal leaves 1-2, long petiole, pentagonal, 2-7cm long, 3-10cm wide. The rhizome is nearly cylindrical, with a maximum diameter of 1 cm, and it can be harvested as medicine in 3 to 5 years. Wild resources are mainly distributed in the damp places under the hillside forests at an altitude of 1500-3000m in the middle and lower reaches of the Yangtze River, and are ...

AI Technical Summary

Problems solved by technology

After summarizing the previous work, it is found that to realize the rapid propagation of the complete plant of Anemone anemone under in vitro culture conditions, so that it can realize the purpose of artificial cultivation, there are still some problems to be solved: (1) the various components of the medium are not very specific, The multiplication efficiency is low; (2) there is a lack of detailed experimental data on biological traits such as reproductive coefficient and growth status of test-tube plantlets; (3) although the rooting rate of test-tube plantlets is high, the regenerated roots are very thin and weak, and the quality of the root system is poor. Affect the absorption of nutrient substances, especially the slow seedling period is longer, and the transplanting survival rate is low; (4) there are no reports in the prior art on hardening and field transplanting of regenerated tree anemone test-tube seedlings, and no more Practical results on the use of in vitro cultured tree anemones for production applications

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