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Detection method of phytoplankton structure

A technology of phytoplankton and community structure, applied in the field of detection of phytoplankton community structure, can solve the problems of destroying the marine ecological balance, endangering human health, fishery and tourism losses, etc.

Inactive Publication Date: 2010-09-22
OCEAN UNIV OF CHINA
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AI Technical Summary

Problems solved by technology

Red tides have seriously damaged the ecological balance of the ocean and caused huge losses to fisheries and tourism.
In addition, some red tide organisms or metabolites contain biological toxins, which can be enriched through the food chain and seriously endanger human health.

Method used

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  • Detection method of phytoplankton structure
  • Detection method of phytoplankton structure
  • Detection method of phytoplankton structure

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Embodiment Construction

[0056] The present invention provides a kind of detection method of phytoplankton community structure, wherein, comprise the following steps:

[0057] a. Phytoplankton water sample collection

[0058] b. Extraction of DNA

[0059] c. PCR and agarose electrophoresis detection

[0060] d. Phytoplankton community structure analysis using denaturing gradient gel electrophoresis

[0061] e. Graphics processing

[0062] f. Data analysis.

[0063] Step a is: the phytoplankton water sample is first filtered with a 200-mesh sieve, and then the filtered seawater is filtered with a nitrocellulose membrane with a pore size of 0.45 μm, and the filter membrane is transferred to a 2ml Eppendorf tube, placed at -20 ° C, with Analysis of the diversity of phytoplankton communities.

[0064] Step b is:

[0065] (1) Collect and wash algal cells: add 750 μl TE buffer to the collected algal cells to wash the algal cells, centrifuge at 10,000 rpm at 4°C for 10 minutes, carefully discard the su...

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Abstract

The invention discloses a detection method of a phytoplankton structure, which comprises the following steps: a, acquiring phytoplankton water samples, b, extracting DNA, c, detecting PCR and agarose by electrophoresis, d, analyzing the phytoplankton structure by a denaturing gradient gel electrophoresis technology, e, processing graphics, and f, analyzing data. In the invention, the PCR product is analyzed by 8% of polyacrylamide gel, the concentration of denaturant is from 35% to 50% (100% denaturant is the mixture of 7M urea and 40% of deionized formamide), and electrophoresis is carried out for 6h in a 1 * TAE buffer solution at 150V; or the PCR product is analyzed by 10% of polyacrylamide gel, the concentration of denaturant is from 30% to 55%, and electrophoresis is carried out for 8h in a 1 * TAE buffer solution at 150V. The invention can accurately detect the structure composition of the phytoplankton, and is conducive to the warning of the outbreak of the phytoplankton red tide.

Description

Technical field: [0001] The invention relates to the technical field of detection of species and / or quantity of marine phytoplankton, in particular to a detection method of phytoplankton community structure. Background technique: [0002] Plankton is a unique group that lives with the current in water bodies, and is easy to move passively under the action of wind and current, with no or only weak swimming ability; plankton includes two categories: phytoplankton and zooplankton. Plankton are small in size, short in life cycle, fast in reproduction, very sensitive to changes in the environment, and can respond quickly to changes in the nutritional status of water bodies. In waters with good water quality, the diversity index and uniformity index of plankton were larger; on the contrary, the species diversity of plankton decreased, and the distribution was uneven. Therefore, changes in the structural characteristics of plankton communities can be used as important biological p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04G01N27/447
Inventor 米铁柱孙静甄毓于志刚
Owner OCEAN UNIV OF CHINA
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