Method for refrigerated storage of heterorhabditis bacteriophora entomopathogenic nematodes in cold areas

Abstract

The invention provides a method for the refrigerated storage of heterorhabditis bacteriophora entomopathogenic nematodes in cold areas, which relates to the technical field of biological refrigerated storage and solves the problems of short storage period, difficult long-distance transportation, low survival rate and low pathogenicity of the conventional low temperature (over 0 DEG C) storage of entomopathogenic nematodes. The method comprises the following steps of: 1) preparing storage media and storage bags; 2) inoculating; 3) performing refrigerated storage at different temperatures; 4) defreezing; and 5) isolating nematodes. The method for the refrigerated storage of the heterorhabditis bacteriophora entomopathogenic nematodes in the cold areas performs the refrigerated storage at the different temperatures on the heterorhabditis bacteriophora entomopathogenic nematodes which are peculiar to high-cold areas, breaks the limitations of current production scale and application areas of the entomopathogenic nematodes to ensure a storage period of 5 to 6 months, a rate of the recovering of the nematodes from the storage medium of 90 percent, a defrozen nematode survival rate of over 80 percent and a soil insect prevention and control effect of over 80 percent, solves the problems of unfavorable long-distance transportation and obviously decreased pathogenicity of the nematodes which are stored at the temperature of over 0 DEG C for long time and achieves a good effect of biologically preventing and controlling destructive insects in agricultural productive practice.

Description

technical field

[0001] The invention relates to the technical field of biological freezing storage, in particular to a variable temperature freezing storage method for Heterorhabditis bacteriophora entomopathogenic nematodes in cold regions. Background technique

[0002] As we all know, entomopathogenic nematodes (Entomopathogenic nematodes) is a kind of potential biological control factor developed in the early 20th century, which refers to a class of parasitic nematodes that carry pathogenic symbiotic bacteria in their bodies and can cause insect pathogenicity. It has been successfully used to control more than 250 kinds of pests such as grubs, mushroom flies, small wood beetles, litchi wood beetles, bald beetles and citrus weevils. Precisely because entomopathogenic nematodes are broad-spectrum, highly efficient, and safe for humans, animals, and the environment, more than 40 countries, including the United States, Australia, Germany, New Zealand, Canada, Japan, Russia, a...