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Cryopreservation method for sperms of ablen

A technology for cryopreservation and fish sperm, which is applied in the field of cryopreservation of grouper sperm, can solve the problems of asynchrony in the sexual maturity of female and male broodstock, damage the interests of farmers, restrict the output of grouper, etc., and achieve low prices. , The cryopreservation method is simple and easy, and the effect is remarkable

Inactive Publication Date: 2010-11-03
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in production practice, the source of male fish is relatively difficult, and the sexual maturity of female and male broodstock is not synchronized, which not only restricts the production of grouper, but also damages the interests of farmers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Ultra-low temperature cryopreservation of the sperm of the grouper

[0026] 1. Sperm collection and microscopic examination: Anesthetize sexually mature males of grouper with eugenol, dry the fish to avoid contact with seawater, and gently press the abdomen to collect semen in a 0.2ml cryopreservation tube. If the viability detected by microscope is above 80%, it can be stored frozen.

[0027] 2. Preparation of antifreeze: take the commonly used balanced salt solution Hank’s as the mother solution, and add dimethyl sulfoxide (DMSO) to a final concentration of 10%.

[0028] 3. Dilution ratio: mix semen with antifreeze, the volume ratio of the two is 1:1.

[0029] 4. Freezing step: equilibrate the cryopreservation tube containing the mixed semen at 4°C for 15 minutes, then place it 6 cm above the liquid nitrogen surface, and balance it for 10 minutes; then balance it on the liquid nitrogen surface for 5 minutes; Store the tubes in liquid nitrogen.

[0030] 5...

Embodiment 2

[0032] Example 2 Ultra-low temperature cryopreservation of saddle grouper sperm

[0033] 1. Semen collection and microscopic examination: Anesthetize the sexually mature male saddle grouper with eugenol, dry the fish body to avoid contact with seawater, and lightly press the abdomen to collect semen in a 0.2ml cryopreservation tube. If the viability detected by microscope is above 80%, it can be stored frozen.

[0034] 2. Preparation of antifreeze: take the commonly used balanced salt solution Hank’s as the mother solution, and add dimethyl sulfoxide (DMSO) to a final concentration of 12%.

[0035] 3. Dilution ratio: mix semen with antifreeze, the volume ratio of the two is 1:1.

[0036] 4. Freezing step: equilibrate the cryopreservation tube containing the mixed semen at 4°C for 15 minutes, then place it 6 cm above the liquid nitrogen surface, and balance it for 10 minutes; then balance it on the liquid nitrogen surface for 5 minutes; Store the tubes in liquid nitrogen. ...

Embodiment 3

[0039] Example 3 Ultra-low temperature cryopreservation of brown spotted grouper sperm

[0040] 1. Sperm collection and microscopic examination: Anesthetize sexually mature males of brown grouper with eugenol, dry the fish body to avoid contact with seawater, and gently press the abdomen to collect semen in a 0.2ml cryopreservation tube. If the viability detected by microscope is above 80%, it can be stored frozen.

[0041] 2. Preparation of antifreeze: take the commonly used balanced salt solution Hank’s as the mother solution, and add dimethyl sulfoxide (DMSO) to a final concentration of 14%.

[0042] 3. Dilution ratio: mix semen with antifreeze, the volume ratio of the two is 1:1.

[0043] 4. Freezing step: equilibrate the cryopreservation tube containing the mixed semen at 4°C for 15 minutes, then place it 6 cm above the liquid nitrogen surface, and balance it for 10 minutes; then balance it on the liquid nitrogen surface for 5 minutes; Store the tubes in liquid nitrog...

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PUM

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Abstract

The invention discloses a cryopreservation method for sperms of ablen, which comprises the following steps of: sampling the sperms, performing microscopic examination, mixing the sperms and an antifreeze agent, and freezing. The cryopreservation method is simple and feasible, has obvious effect and good cryopreservation effect. If the sperms of the ablen most important in Chinese aquiculture are preserved by the method, the activity of the unfrozen sperms is more than 90 percent, and the bottleneck problems of insufficient male fish and asynchronous sexual maturity of female and male parent fish in the ablen culture are effectively solved.

Description

technical field [0001] The invention relates to the field of sperm preservation, in particular to a cryopreservation method for grouper sperm. Background technique [0002] Grouper is a world-renowned rare marine fish with delicious meat, rich nutrition and high economic value. my country's Guangdong, Hainan, Fujian and other provinces have carried out grouper farming. Some species such as saddle grouper and oblique grouper have been rapidly popularized and promoted artificially, and have become important marine culture species in the southern coast. [0003] The grouper fish has the phenomenon of "female first, then male". It is a hermaphroditic female prematurity fish, and the sex conversion of senior fish (3-6 years old) turns into male fish. Therefore, in production practice, the source of male fish is relatively difficult, and the sexual maturity of female and male broodstock is not synchronized, which not only restricts the production of grouper, but also damages the ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 蒙子宁范斌刘晓春张海发张勇林浩然
Owner SUN YAT SEN UNIV
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