The invention relates to an application of a rubber seedcake to an oncorhynchus mykiss mixed feed and a formula of the feed, and belongs to the field of aquaculture techniques. The fixed feed comprises the following components by weight percentage: 15-20% of fish meal, 6-10% of corn protein powder, 7-10% of soybean protein concentrate, 13-18% of bean dregs, 9-12% of rapeseed dregs, 7-10% of cottonseed dregs, 5-15% of the rubber seedcake, 1-7% of wheat middling, 10-12% of bean oil, 0.3-0.5% of soybean lecithin, 0.7-1.5% of monocalcium phosphate, 0.05-0.1% of vitamin C phosphate, 0.1-0.2% of choline chloride, 0.01-0.03% of ethoxy quinoline, 0.5-1.0% of a mineral premix compound, and 0.5-1.0% of a vitamin premix compound. According to the application and the formula, short protein sources such as the bean dregs, the cottonseed dregs and the rapeseed dregs are saved; the cost of the formula and the coefficient of the feed are lowered and reduced; the feed has a high cost performance; the aquiculture benefit of oncorhynchus mykiss is improved.