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Concentrated probiotics preparation and production thereof

A probiotic preparation and a probiotic technology are applied in the field of concentrated probiotic preparations and their preparation, which can solve the problems of inability to inhibit and kill Bacillus propagules and molds, high production cost, short shelf life, etc. The effect of low production cost and stable quality

Inactive Publication Date: 2006-08-16
SANJIANG PLASTIC AUXILIARY LIYANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This preparation method has high production cost, and the bacteria after high-speed centrifugation will become fragile and cause death
The shelf life is short, and long-term storage will cause damage to the finished product due to the reproduction of residual bacteria and spores
This product is not suitable for long-distance transportation, especially it cannot inhibit and kill the bacillus propagule and mold caused by pollution during long-term storage and production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] a. Making probiotic activating solution: select photosynthetic bacteria, Lactobacillus acidophilus, bifidobacterium and cultured Bacillus cereus spores mixed together as the activating strain. Then choose dipotassium hydrogen phosphate 1g, magnesium chloride 1g, calcium chloride 2g, sodium propionate 2g and dissolve them in 1000ml distilled water, then add 1.5g vitamin C, vitamin E and 0.5g magnesium sulfate, calcium carbonate, stir well and Adjust the pH value to 6.5-7.0 to make the culture medium. Add 250 g of activated bacteria into 750 g of culture medium and culture at 30° C. for 105 hours to prepare 1000 g of probiotic activation solution.

[0036] b. Preparation of bacterial liquid flocculant: fully mix 3 g of chitosan and 3 g of alum to prepare 6 g of bacterial liquid flocculant.

[0037] c. Make protective agent: Add 20g of skimmed milk powder into 1000ml of distilled water and fully dissolve it, then add 5g of brown sugar, heat the above concentrated solution...

Embodiment 2

[0040] a. Making probiotic activating liquid: choose photosynthetic bacteria as the activating strain. Choose 0.5g of dipotassium hydrogen phosphate, 1.5g of magnesium chloride, 1.5g of calcium chloride, 2.5g of sodium propionate and dissolve them in 800ml of distilled water, then add 1g of vitamin C, vitamin E and 0.8g of calcium carbonate and zinc chloride. Stir evenly and adjust the pH value to 6.5-7.0 to make the culture medium. Add 200 g of activated bacteria into 800 g of culture medium and culture at 25° C. for 120 hours to prepare 1000 g of probiotic activation solution.

[0041] b. Preparation of bacterial liquid flocculant: fully mix 2.4g of chitosan and 3.6g of alum to prepare 6g of bacterial liquid flocculant.

[0042] c. Make protective agent: Add 15g of skimmed milk powder into 800ml of distilled water and dissolve it fully, then add 7g of brown sugar, heat the above concentrated solution to 50°C, finally add 15g of glycerin and mix well, wait to cool to room te...

Embodiment 3

[0045]a. Making probiotic activating liquid: select the compound strains mixed with Lactobacillus acidophilus, Bifidobacterium and cultured Bacillus licheniformis spores as the activated strains. Then choose 1.5g of dipotassium hydrogen phosphate, 0.5g of magnesium chloride, 2.5g of calcium chloride, 1.5g of sodium propionate and dissolve them in 1200ml of distilled water, then add 1.2g of vitamin E, vitamin C and 0.7g of calcium carbonate, and stir well And adjust the pH value to 6.5-7.0 to make the culture medium. Add 300 g of activated bacteria into 700 g of culture medium and culture at 40° C. for 96 hours to prepare 1000 g of probiotic activation solution.

[0046] b. Preparation of bacterial fluid flocculant: fully mix 3.6g of chitosan and 2.4g of alum to prepare 6g of bacterial fluid flocculant.

[0047] c. Make protective agent: Add 25g of skimmed milk powder into 1200ml of distilled water and dissolve it fully, then add 6g of brown sugar, heat the above concentrated ...

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PUM

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Abstract

An enrichment probiotics pharmaceutics and its preparation method are provided in the invention. The medium is made up of the phosphate, the MgCl2, the CaCl2, the sodium propionate, the distilled water, the proper growth factor and the microelement. And its pH is adjusted to the proper. Then the actived strain is added into the medium to culture. The flocculating agent is made up of the chitosan and the alum. The protecting agent is made up of the dried skim milk, the brown sugar, the glycerol, the distilled water. The weight ration of the probiotics pharmaceutics is probiotics liquid 800-1200 portion, flocculating agent 1-25 portion, the protecting agent 80-120 portion. It can be used to the aquiculture, the agricultural plant, the effluent treatment and the fancy fish breeding. It can inhibit or kill the breeding of the bacillus and the fungus.

Description

Technical field [0001] The invention relates to a concentrated probiotic preparation and a preparation method thereof. Background technique [0002] Most of the probiotic preparations currently on the market, especially those used in aquaculture, are relatively dilute liquid preparations. During the preparation process, the medium needs to be sterilized at high temperature and the supernatant should be discarded by a high-speed centrifuge. . The production cost of this preparation method is high, and the thallus after high-speed centrifugation will become fragile and cause death. The shelf life is short, and long-term storage will cause loss of finished products due to the reproduction of residual bacteria and their spores. This product is also not suitable for long-distance transportation, especially cannot inhibit and kill the effect of bacillus propagule and mold produced due to pollution during long-term storage and production. Contents of the invention [0003] The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20A23L1/29A23L33/00
Inventor 王毅谦史云飞陆上苏陆剑平
Owner SANJIANG PLASTIC AUXILIARY LIYANG
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