Biological preparation for inhibiting growth of vibrios in shrimp ponds and preparation method and application thereof

A biological agent and Vibrio technology, applied in the field of aquaculture water quality control, can solve the problems of not being able to adapt to the nutrient environment of aquaculture wastewater, poor resistance to adverse environmental impacts, and unstable treatment effects, so as to improve the body's immunity, efficiently and safely ammonia nitrogen Harm, improve the effect of water safety

Active Publication Date: 2018-12-21
广州海诚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, biological contact oxidation (Liao Shaoan et al., 2008), biological turntable (Brazil, 2006), biological fluidized bed (Bortone et al, 2003) and other processes are widely used in the biochemical treatment of aquaculture wastewater. In these processes, the biological phase is mostly It is derived from oligotrophic flora in natural or artificial environments, and cannot adapt to the nutritional environment of aquaculture wastewater. The unit volume processing load of the microbial treatment module is actually not high, which greatly affects the application of traditional microbial treatment technologies.
[0006] At present, single or compound microbial strains are generally used to control water quality at home and abroad, but because microorganisms are greatly affected by the external environment and have poor resistance to adverse environmental impacts, once the system is damaged, it is difficult to recover, so the treatment effect is unstable.

Method used

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  • Biological preparation for inhibiting growth of vibrios in shrimp ponds and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Preparation of starter

[0048] 1) Preparation of activation medium

[0049] The activation medium used for starter activation includes the following components by weight: 1000 parts of aquaculture pond water, 20 parts of brown sugar, 0.5 parts of ammonium sulfate, 0.05 parts of magnesium sulfate heptahydrate, 0.5 parts of dipotassium hydrogen phosphate, and 14.29 parts of calcium carbonate. 2 parts of sodium chloride, 1 part of brine, 0.02 parts of calcium chloride monohydrate, 0.05 parts of ferrous sulfate heptahydrate, 0.05 parts of copper sulfate pentahydrate.

[0050] 2) Activation of complex bacteria A:

[0051] The activation medium prepared by adding 1) adopts aerobic activation, and culturing at 30°C for 36 hours for activation to obtain fermentation broth A.

[0052] 3) Activation of complex bacteria B:

[0053] The activation medium obtained by adding 1) adopts a sealed activation method, and the fermentation broth B is obtained by culturing at 30° C. for 36 hours f...

Embodiment 2

[0061] 1. Preparation of starter

[0062] 1) Preparation of activation medium

[0063] The activation medium used for starter activation includes the following components by weight: 1000 parts of aquaculture pond water, 20 parts of brown sugar, 0.5 parts of ammonium sulfate, 0.05 parts of magnesium sulfate heptahydrate, 0.5 parts of dipotassium hydrogen phosphate, and 14.29 parts of calcium carbonate. 2 parts of sodium chloride, 1 part of brine, 0.02 parts of calcium chloride monohydrate, 0.05 parts of ferrous sulfate heptahydrate, 0.05 parts of copper sulfate pentahydrate.

[0064] 2) Activation of complex bacteria A:

[0065] The activation medium obtained by adding 1) adopts aerobic activation, and culturing at 27° C. for 48 hours for activation to obtain fermentation broth A.

[0066] 3) Activation of composite bacteria B: adding 1) the prepared activation medium adopts a sealed activation method, cultured at 27°C for 48 hours for activation, and fermentation broth B is obtained.

...

Embodiment 3

[0074] 1. Preparation of starter

[0075] 1) Preparation of activation medium

[0076] The activation medium used for starter activation includes the following components by weight: 1000 parts of aquaculture pond water, 20 parts of brown sugar, 0.5 parts of ammonium sulfate, 0.05 parts of magnesium sulfate heptahydrate, 0.5 parts of dipotassium hydrogen phosphate, and 14.29 parts of calcium carbonate. 2 parts of sodium chloride, 1 part of brine, 0.02 parts of calcium chloride monohydrate, 0.05 parts of ferrous sulfate heptahydrate, 0.05 parts of copper sulfate pentahydrate.

[0077] 2) Activation of complex bacteria A:

[0078] The activation medium prepared by adding 1) adopts aerobic activation, and culturing at 30°C for 24 hours for activation to obtain fermentation broth A.

[0079] 3) Activation of complex bacteria B:

[0080] The activation medium obtained by adding 1) adopts a sealed activation method, and culturing at 30° C. for 24 hours for activation to obtain fermentation bro...

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Abstract

The invention provides a starter culture for a biological preparation for inhibiting the growth of vibrios in shrimp ponds. The starter culture comprises the following bacterial contents accounting for total bacteria count: 5 to 15 percent of bdellovibrio bacteriovorus, 5 to 15 percent of bdellovibrio starrii, 20 to 40 percent of bacillus, 20 to 40 percent of lactobacillus and 10 to 30 percent ofrhodotorula benthica. The invention further provides a biological preparation for inhibiting the growth of vibrios in shrimp ponds and a preparation method thereof, and the biological preparation is mainly prepared by activating the starter culture to ferment material. According to the biological preparation, by reasonably matching multiple probiotic agents to ferment the material to prepare the biological preparation, the mutual cooperation between the different strains is utilized to obtain the biological preparation capable of effectively inhibiting the growth of vibrios in the shrimp ponds, and the preparation method for the preparation is simple, does not have high requirement on equipment, and has high operability, and the biological preparation can be easily popularized for use in large-scale aquafarms or aquiculture cooperatives.

Description

Technical field [0001] The invention relates to the field of aquaculture water quality regulation and control, in particular to a biological preparation capable of inhibiting the growth of Vibrio in shrimp ponds, and a preparation method and application thereof. Background technique [0002] Shrimp farming is an important pillar industry in my country's aquaculture industry. However, in recent years, with the increase in aquaculture production, the increase in aquaculture density, the large-scale development of shrimp aquaculture, and the intensified pollution of aquaculture, the quality of the aquaculture environment has continued to deteriorate, resulting in an increase in the number of pathogenic organisms and the acceleration of the spread, and the severity of aquaculture diseases. Diseases in shrimp farming, especially bacterial diseases, occur frequently and have serious impacts, causing huge economic losses and greatly restricting the development of my country's aquaculture...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C02F103/20C12R1/63C12R1/07C12R1/645
CPCC02F3/34C02F2103/20C12N1/16C12N1/20
Inventor 巩华陈克松焦彩虹
Owner 广州海诚生物科技有限公司
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