A biological agent capable of inhibiting the growth of Vibrio in shrimp ponds, its preparation method and application

A biological agent and Vibrio technology, applied in the field of aquaculture water quality control, can solve the problems of not being able to adapt to the nutrient environment of aquaculture wastewater, poor resistance to adverse environmental impacts, and unstable treatment effects, so as to improve the body's immunity, efficiently and safely ammonia nitrogen Harm, improve the effect of water safety

Active Publication Date: 2021-10-01
广州海诚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, biological contact oxidation (Liao Shaoan et al., 2008), biological turntable (Brazil, 2006), biological fluidized bed (Bortone et al, 2003) and other processes are widely used in the biochemical treatment of aquaculture wastewater. In these processes, the biological phase is mostly It is derived from oligotrophic flora in natural or artificial environments, and cannot adapt to the nutritional environment of aquaculture wastewater. The unit volume processing load of the microbial treatment module is actually not high, which greatly affects the application of traditional microbial treatment technologies.
[0006] At present, single or compound microbial strains are generally used to control water quality at home and abroad, but because microorganisms are greatly affected by the external environment and have poor resistance to adverse environmental impacts, once the system is damaged, it is difficult to recover, so the treatment effect is unstable.

Method used

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  • A biological agent capable of inhibiting the growth of Vibrio in shrimp ponds, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Preparation of starter

[0048] 1) Preparation of activated medium

[0049] The used activation medium for the activation of the starter comprises the following components by weight: 1000 parts of culture pond water, 20 parts of brown sugar, 0.5 part of ammonium sulfate, 0.05 part of magnesium sulfate heptahydrate, 0.5 part of dipotassium hydrogen phosphate, 14.29 parts of calcium carbonate, 2 parts of sodium chloride, 1 part of brine, 0.02 part of calcium chloride monohydrate, 0.05 part of ferrous sulfate heptahydrate, and 0.05 part of copper sulfate pentahydrate.

[0050] 2) Activation of complex bacteria A:

[0051] Add the activation medium prepared in 1) by means of aerobic activation, and activate it by culturing at 30°C for 36 hours to obtain fermentation broth A.

[0052] 3) Activation of complex bacteria B:

[0053] Add the activation medium prepared in 1) by sealing and activating, culture at 30° C. for 36 hours for activation, and obtain fermentation br...

Embodiment 2

[0061] 1. Preparation of starter

[0062] 1) Preparation of activated medium

[0063] The used activation medium for the activation of the starter comprises the following components by weight: 1000 parts of culture pond water, 20 parts of brown sugar, 0.5 part of ammonium sulfate, 0.05 part of magnesium sulfate heptahydrate, 0.5 part of dipotassium hydrogen phosphate, 14.29 parts of calcium carbonate, 2 parts of sodium chloride, 1 part of brine, 0.02 part of calcium chloride monohydrate, 0.05 part of ferrous sulfate heptahydrate, and 0.05 part of copper sulfate pentahydrate.

[0064] 2) Activation of complex bacteria A:

[0065] Add the activation medium prepared in 1) by means of aerobic activation, and activate it by culturing at 27°C for 48 hours to obtain fermentation broth A.

[0066] 3) Activation of complex bacteria B: add the activation medium prepared in 1) by sealing and activating, culture at 27° C. for 48 hours for activation, and obtain fermentation broth B.

[0...

Embodiment 3

[0074] 1. Preparation of starter

[0075] 1) Preparation of activated medium

[0076] The used activation medium for the activation of the starter comprises the following components by weight: 1000 parts of culture pond water, 20 parts of brown sugar, 0.5 part of ammonium sulfate, 0.05 part of magnesium sulfate heptahydrate, 0.5 part of dipotassium hydrogen phosphate, 14.29 parts of calcium carbonate, 2 parts of sodium chloride, 1 part of brine, 0.02 part of calcium chloride monohydrate, 0.05 part of ferrous sulfate heptahydrate, and 0.05 part of copper sulfate pentahydrate.

[0077] 2) Activation of complex bacteria A:

[0078] Add the activation medium prepared in 1) by means of aerobic activation, culture at 30° C. for 24 hours for activation, and obtain fermentation broth A.

[0079] 3) Activation of complex bacteria B:

[0080] Add the activation medium prepared in 1) by sealing and activating, culture at 30° C. for 24 hours for activation, and obtain fermentation brot...

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Abstract

The invention provides a starter with a biological agent for inhibiting the growth of Vibrio in shrimp ponds. Bdellovibrio thaler, 20% to 40% of Bacillus, 20% to 40% of lactic acid bacteria and 10% to 30% of marine red yeast. The present invention also extracts a biological preparation capable of inhibiting the growth of Vibrio in shrimp ponds and a preparation method thereof. The biological preparation is mainly prepared from the fermentation raw material after activation of the above-mentioned starter. The present invention prepares biological preparations by rationally matching a variety of probiotic fermentation raw materials, and utilizes the mutual cooperation of different strains to obtain a biological preparation that can effectively inhibit the growth of Vibrio in shrimp ponds. The preparation method of the preparation is simple and does not require much equipment. High, operability, easy to promote and use in large-scale farms or aquaculture cooperatives.

Description

technical field [0001] The invention relates to the field of aquaculture water quality control, in particular to a biological agent capable of inhibiting the growth of Vibrio in shrimp ponds, a preparation method and application thereof. Background technique [0002] Shrimp farming is an important pillar industry in my country's aquaculture industry. However, in recent years, with the increase of aquaculture production, the increase of aquaculture density, the large-scale development of prawn aquaculture, and the aggravation of aquaculture pollution, the quality of aquaculture environment has continued to deteriorate, leading to an increase in the number of pathogenic organisms and a faster transmission speed, and the aquaculture diseases are becoming more and more serious. Diseases in shrimp farming, especially bacterial diseases, occur frequently and have serious impacts, causing huge economic losses and greatly limiting the development of aquaculture in my country. Breedi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C02F103/20C12R1/63C12R1/07C12R1/645
CPCC02F3/34C02F2103/20C12N1/16C12N1/20
Inventor 巩华陈克松焦彩虹
Owner 广州海诚生物科技有限公司
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