Method for detecting mononucleotide polymorphism of scalper SREBP1c gene

Abstract

The invention discloses a method for detecting mononucleotide polymorphism of a scalper SREBP1c gene, which comprises the following steps of: using a scalper whole genome DNA to be detected containing the SREBP1c gene as a template, using a primer pair P as a primer, and performing PCR amplification on the scalper SREBP1c gene; using a restriction endonuclease Bg1 I to digest a PCR amplification product, and then performing sepharose gel electrophoresis on an amplified fragment after enzyme digestion; and identifying the mononucleotide polymorphism of the 10914th bit of the scalper SREBP1c gene according to the result of the sepharose gel electrophoresis. Because the functions of the SREBP1c gene relate to four important growth traits of body weight, daily gain, body length and chest circumference, the detection method provided by the invention lays a good foundation for establishing a relation between SNP and the growth traits of the SREBP1c gene, and is convenient to be used in a marker assistant selection (MAS) of the growth traits of Chinese scalper beef to quickly establish a scalper population with an excellent genetic resource.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the single nucleotide polymorphism of cattle SREBP1c (sterol regulatory element binding protein 1c) gene. Background technique [0002] Gene polymorphism refers to the differences in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, mainly including base substitutions, insertions, deletions and duplications. Changes in sequence copy number. [0003] Single Nucleotide Polymorphism (SNP) is a type of genetic marker system proposed by Lander (1996), a scholar at the Human Genome Research Center of the Massachusetts Institute of Technology. Polymorphism due to acid (A / T / C / G) substitution. Its variant forms are: transversion, conversion, insertion and deletion, etc., ma...

AI Technical Summary

Problems solved by technology

At present, there is a lack of research in the field of genetic variation of the SREBP1c gene in cattle. The functional study of this gene locus and its genetic variation and growth and development traits (such as: birth weight, body weight, daily gain, body height, body oblique length, chest circumference, ischial end width, etc. Traits) association research is still blank

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