Method for detecting mononucleotide polymorphism of scalper SREBP1c gene

A single nucleotide polymorphism, gene technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of lack of research and blank, and achieve the effect of excellent genetic resources

Inactive Publication Date: 2010-11-03
NORTHWEST A & F UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of research in the field of genetic variation of the SREBP1c gene in cattle. The functional study of this gene locus and its genetic variation and growth and development traits (such as: birth weight, body weight, daily gain, body height, body oblique length, chest circumference, ischial end width, etc. Traits) association research is still blank

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting mononucleotide polymorphism of scalper SREBP1c gene
  • Method for detecting mononucleotide polymorphism of scalper SREBP1c gene
  • Method for detecting mononucleotide polymorphism of scalper SREBP1c gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The invention detects the single nucleotide polymorphism of the 10914th position missense mutation of the yellow cattle SREBP1c gene that may produce a change in the conformation of the encoded protein, so as to be used for the marker-assisted selection of the growth traits of Chinese yellow beef, and rapidly establish genetic resources Excellent cattle population. The present invention will be further described in detail below in conjunction with the detection of specific samples and the correlation of traits, which are to explain rather than limit the present invention.

[0030] a. Design of PCR primers containing nt10914 mutation in cattle SREBP1c gene

[0031]Taking the sequence of Hereford cattle (NC_NC_007317.3) published by NCBI as a reference, using Primer5.0 to design PCR primers that can amplify the region of exon 9 of cattle SREBP1c gene, the primer sequences are as follows:

[0032] Upstream primer F1: cgact acatc cgctt cctt 19;

[0033] Downstream primer ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for detecting mononucleotide polymorphism of a scalper SREBP1c gene, which comprises the following steps of: using a scalper whole genome DNA to be detected containing the SREBP1c gene as a template, using a primer pair P as a primer, and performing PCR amplification on the scalper SREBP1c gene; using a restriction endonuclease Bg1 I to digest a PCR amplification product, and then performing sepharose gel electrophoresis on an amplified fragment after enzyme digestion; and identifying the mononucleotide polymorphism of the 10914th bit of the scalper SREBP1c gene according to the result of the sepharose gel electrophoresis. Because the functions of the SREBP1c gene relate to four important growth traits of body weight, daily gain, body length and chest circumference, the detection method provided by the invention lays a good foundation for establishing a relation between SNP and the growth traits of the SREBP1c gene, and is convenient to be used in a marker assistant selection (MAS) of the growth traits of Chinese scalper beef to quickly establish a scalper population with an excellent genetic resource.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the single nucleotide polymorphism of cattle SREBP1c (sterol regulatory element binding protein 1c) gene. Background technique [0002] Gene polymorphism refers to the differences in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, mainly including base substitutions, insertions, deletions and duplications. Changes in sequence copy number. [0003] Single Nucleotide Polymorphism (SNP) is a type of genetic marker system proposed by Lander (1996), a scholar at the Human Genome Research Center of the Massachusetts Institute of Technology. Polymorphism due to acid (A / T / C / G) substitution. Its variant forms are: transversion, conversion, insertion and deletion, etc., ma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈宏黄永震淮永涛张宝李转见蓝贤勇胡沈荣雷初朝
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap