Preparation process for Sappan Wood extract perfusate and application thereof in treating bladder cancer

A preparation process and perfusate technology, which can be used in drug combinations, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of BCG with large toxic and side effects, cannot be used immediately, and affect tumor cell planting, etc., and achieve good therapeutic effects. , the effect of remarkable curative effect and good development prospect

Inactive Publication Date: 2010-12-08
山西华尚汇商贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high toxicity and side effects of BCG, it cannot be used immediately after tumor resection, thus affecting the effect of preventing tumor cell implantation
[00

Method used

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  • Preparation process for Sappan Wood extract perfusate and application thereof in treating bladder cancer
  • Preparation process for Sappan Wood extract perfusate and application thereof in treating bladder cancer
  • Preparation process for Sappan Wood extract perfusate and application thereof in treating bladder cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Embodiment 1, a preparation process of bladder perfusion solution, the steps include: take hematoxylin and pulverize it, add 5 times the weight of purified water for the first time, boil and extract for 60 minutes to filter to obtain the filtrate, then add 3 times the weight of purified water for the second time, boil Extract for 40 minutes and filter to obtain the filtrate, then add 2 times the weight of purified water for the third time, boil and extract for 30 minutes and combine the extracts obtained three times, concentrate under reduced pressure to 2 times the weight of the crude drug; let stand overnight to remove the precipitate; then add a Double the volume of petroleum ether, extract, and keep the water phase; add three times the volume of ethyl acetate, extract, remove the water phase; evaporate the ethyl acetate under reduced pressure; weigh the dry matter, add purified water, and make the dry matter The content is 2%, heating and dissolving. Let it stand at...

Embodiment 2

[0095] Embodiment 2, a preparation process of bladder perfusion solution, the steps include: taking hematoxylin and pulverizing it, adding 3 times the amount of purified water for the first time, boiling and extracting for 65 minutes to filter to obtain the filtrate, then adding 2 times the amount of purified water for the second time, boiling Extract for 50 minutes and filter to obtain the filtrate, then add 1.5 times the amount of purified water for the third time, boil and extract for 35 minutes; combine the extracts obtained three times, and concentrate under reduced pressure to 2 times the crude drug amount of Sappan; let stand overnight to remove the precipitate; Then add twice the amount of petroleum ether, extract, and keep the water phase; add four times more ethyl acetate, extract, and remove the water phase; evaporate the ethyl acetate under reduced pressure; weigh the dry matter, add purified water, and make the dry matter content 2%, heat to dissolve, stand at room...

Embodiment 3

[0096] Embodiment 3, a preparation process of bladder perfusion solution, the steps include: taking hematoxylin and pulverizing it, adding 6 times the amount of purified water for the first time, boiling and extracting for 45 minutes to filter to obtain the filtrate, then adding 4 times the amount of purified water for the second time, boiling Extract for 30 minutes and filter to obtain the filtrate, then add 2.5 times the amount of purified water for the third time, boil and extract for 25 minutes; combine the extracts obtained three times, concentrate under reduced pressure to 2 times the amount of the crude drug of sumacin; let stand overnight to remove the precipitate; Then add 1.5 times the amount of petroleum ether, extract, and keep the water phase; then add 2 times the amount of ethyl acetate, extract, and remove the water phase; evaporate the ethyl acetate under reduced pressure; weigh the dry matter, add purified water, and make the dry matter The content is 2%, disso...

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Abstract

The invention relates to a preparation method for a Sappan Wood extract, in particular to a preparation process for Sappan Wood extract perfusate and application thereof in treating bladder cancer, which solves the problem of the application of the Sappan Wood extract in treating bladder cancer tumors. The preparation process comprises the following steps: taking and smashing Sappan Wood, carrying out water extracting for three times, combining the extracting solutions obtained by three-time water extracting, and carrying out decompression concentrating; standing overnight, and removing sediments; adding petroleum ether, extracting, and retaining aqueous phase; adding ethyl acetate, extracting, and removing the aqueous phase; carrying out decompression volatilizing to remove all the ethyl acetate; weighing dry substances, adding purified water to enable the dry substance content to be 2%, heating for dissolving, standing overnight at room temperature, and filtering out sediments; and freeze-drying, and carrying out split charging. The experiment research result shows that the extract has obvious killing effect on bladder neoplasm cells; and compared with mitomycin, epirubicin, Pirarubicin and other anticancer drugs, which are clinically and frequently used, the Sappan Wood extract perfusate has the advantages of high anticancer activity, less toxicity, safety, effectiveness and the like.

Description

technical field [0001] The invention relates to a preparation method of sumac extract, in particular to a preparation process of sumac extract perfusate and its application in treating bladder cancer. Background technique [0002] Sumac (Caesalpinia sappan Linn.), also known as Sufang, Fangfangmu and Sufang. The hematoxylin heartwood can be used as medicine, and it is mostly harvested in autumn. The white sapwood is removed and dried. The dried heartwood is long cylindrical or half-cylindrical, with a length of 10-100 cm and a diameter of 3-13 cm. The surface is yellow-red to brown-red, with knife-cut marks and common longitudinal cracks. Quality is hard. The cross-section is slightly shiny, with obvious annual rings, and some can be seen with dark brown, pine, and bright star pith, with a slight smell and a slightly astringent taste. Nature and flavor are sweet, salty, flat. GUIXIN, liver, spleen channel. It has the effects of promoting blood circulation and removing b...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P35/00
Inventor 王国平王晋芬任连生张蕻
Owner 山西华尚汇商贸有限公司
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