Drug composition with anti-tumor effect
A technology with anti-tumor effect and anti-inflammatory effect, which is applied in the direction of anti-tumor drugs, drug combinations, active ingredients of hydroxyl compounds, etc. It can solve problems such as difficult to use anti-tumor drugs, cancer synergies are not often found, and achieve inhibition Tumor growth, anti-tumor effect on tumor growth
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] Example 1: Combination of Elemene and Celecoxib on Human Hep G 2 inhibition of cells
[0039] Material :
[0040] Cell line Human liver cancer cell line Hep G 2 (purchased from Shanghai Cell Bank)
[0041] Drug solution preparation: Elemene is dissolved in DMSO before use, and then added to cultured cells to prepare the desired final concentration of the drug. Celecoxib was dissolved in DMSO to prepare mother solutions of various concentrations, and stored in a refrigerator. During the experiment, it was added to the cultured cells to prepare the desired final concentration of the drug.
[0042] Cell culture: human liver cancer cell line Hep G 2 Inoculate in a sterile culture bottle, add an appropriate amount of DMEM culture solution, and incubate at 37°C in 5% CO 2 cultured in an incubator with saturated humidity. The cells grow adherently in a monolayer and are passaged every 2-3 days. Digest and culture the cells with 0.25% trypsin + 0.02% EDTA for 2-4 minut...
Embodiment 2
[0047] Example 2: Detection of apoptosis
[0048] Hep G in logarithmic growth phase 2 cells, made into a single cell suspension, at 1×10 5 / ml inoculated in a 6-well plate, 2 ml per well, after the cells adhered to the wall, different concentrations of drugs were added, and the cells were collected after continuing to culture for 24 hours. Transfer to a centrifuge tube, centrifuge at 1000 r / min for 3 min, discard the supernatant, wash with incubation buffer solution, add 100 ml of labeling solution and disperse the cells with a 400-mesh nylon mesh, incubate at room temperature for 15 min in the dark, centrifuge at 800 rpm for 5 min, and rinse with incubation buffer After washing, add Annexin V / PI staining solution and incubate at 4°C for 20min, and then detect on the machine. The excitation light wavelength of the flow cytometer is 488 nm, and a filter with a wavelength of 515 nm is used to detect FITC fluorescence, and another filter with a wavelength greater than 560 nm is...
Embodiment 3
[0050] Embodiment 3: AO / EB fluorescent staining
[0051] Take the cells in the logarithmic growth phase, make a single cell suspension according to the subculture method, and count them at 1 × 10 5 / ml density in six-well plates. Place the culture plate at 37°C, 5% CO 2 After 24 hours of adherent culture in the incubator, add different concentrations of drugs to each well, and continue to incubate for 24 hours; collect all the cells, transfer them to a centrifuge tube, centrifuge at 1000 rpm for 3 minutes, wash twice with PBS, and add 2ml PBS to resuspend the cells , take 100 ml of cell suspension into EP tube, add 8 ml of AO / EB staining solution, and incubate for 15 min. Under the microscope, green light can stimulate EB to observe late apoptosis, and blue light can stimulate AO to observe early apoptosis.
[0052] The results showed that under the microscope the cells in the combined drug group were significantly shrunken and rounded, with blurred shape, chromatin condens...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap