Biological tissue dehydrator and application thereof

A technology of biological tissue and dehydrator, which is applied in the field of biopathological tissue detection, can solve the problems of complex mechanical design and operation, instrument stability deviation, and slow dehydration speed, so as to protect the health of the body, prevent damage, and maintain the original traits Effect

Inactive Publication Date: 2010-12-22
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the dehydration speed of this method is slow, the sudden concentration will cause certain damage to the tissue, the volatilized solvent has a potential carcinogenic threat to the operator, and the complicated mechanical design and operation lead to deviations in the stability of the instrument
There is currently no efficient tissue dehydration and transparent special device for tissue dehydration by continuously changing the concentration of dehydrating agent or clearing agent in the market, so the device of the present invention can fill the gap in this field

Method used

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  • Biological tissue dehydrator and application thereof
  • Biological tissue dehydrator and application thereof
  • Biological tissue dehydrator and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, take a suitable size tissue piece, the diameter should be less than 7mm, use a double-sided blade to trim the tissue piece to 3~5mm 3 size. After being fixed with formaldehyde, it is used for dehydration and transparency.

[0021]A) In the dehydration and transparent process of the traditional method, it is required to shake the tissue block frequently to ensure that the tissue block can be fully contacted with ethanol or xylene. After each step, the tissue block should be fully dried, usually with a paper tube Blot the liquid on the tissue block so as not to affect the concentration of other liquids, but to prevent the tissue block from drying out, fill each liquid cylinder with 100 ml of liquid. The whole process takes about 5h (300min). The color of the tissue block is darkened and transparent, and the color of the xylene solution is transparent. There is a clear smell of solvent in the operating environment. The operation of the traditional method ...

Embodiment 2

[0027] Embodiment 2, get the tissue piece of suitable size, diameter should be less than 7mm, use double-sided blade to trim the tissue piece to 3 ~ 5mm 3 size. After being fixed with formaldehyde, it is used for dehydration and transparency.

[0028] A) In the dehydration and transparent process of the traditional method, it is required to shake the tissue block frequently to ensure that the tissue block can be fully contacted with ethanol or xylene. After each step, the tissue block should be fully dried, usually with a paper tube Blot the liquid on the tissue block so as not to affect the concentration of other liquids, but to prevent the tissue block from drying out, fill each liquid cylinder with 100 ml of liquid. The whole process takes about 5h (300min). The color of the tissue block is darkened and transparent, and the color of the xylene solution is transparent. There is a clear smell of solvent in the operating environment. The operation of the traditional method...

Embodiment 3

[0030] Embodiment 3, get the tissue piece of suitable size, diameter should be less than 7mm, use double-sided blade to trim the tissue piece into 3 ~ 5mm 3 size. After being fixed with formaldehyde, it is used for dehydration and transparency.

[0031] A) In the dehydration and transparent process of the traditional method, it is required to shake the tissue block frequently to ensure that the tissue block can be fully contacted with ethanol or xylene. After each step, the tissue block should be fully dried, usually with a paper tube Blot the liquid on the tissue block so as not to affect the concentration of other liquids, but to prevent the tissue block from drying out, fill each liquid cylinder with 100 ml of liquid. The whole process takes about 5h (300min). The color of the tissue block is darkened and transparent, and the color of the xylene solution is transparent. There is a clear smell of solvent in the operating environment. The operation of the traditional meth...

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Abstract

The invention relates to a dehydrated method using a biological tissue dehydrator, belonging to the field of biological pathology detection. The biological tissue dehydrator is a continuous feeding and discharging airtight sample slot. In the dehydrating mode of the dehydrator, a biological pathology tissue is fixed in the sample slot, and dehydrating agents the concentrations of which are from low to high are continuously introduced to carry out dehydration. In the process of tissue dehydration or transparency, the speed of molecular diffusion is increased, the tissue dehydration and transparency speed is accelerated and the high-efficiency dehydration and transparency of the pathology tissue are realized according to a diffusion theory through stirring or supersonic vibration in the sample slot. The invention has important facilitation on promoting the speed and the efficiency of biological pathology tissue detection.

Description

technical field [0001] The invention belongs to the field of biological pathological tissue detection, and relates to a biological tissue dehydrator and its application. Background technique [0002] Biological tissue will quickly decay after leaving the living body, losing its original normal structure, and cannot be used for tissue observation and research. Paraffin-fixed tissues are preserved and used for section observation, which is the most widely used method in biological histology. Through paraffin sectioning, the morphological structure of cells and biological tissues can be observed under a microscope. It is the main method for studying, observing and judging the morphological changes of cells and tissues and the distribution of biological macromolecules in biology, pathology and forensic science. In vitro observation of tissue requires steps such as fixation, paraffin embedding, sectioning, and staining to achieve long-term preservation of the tissue. [0003] A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/40
Inventor 孟春王航郭养浩周堃陈雄峰
Owner FUZHOU UNIV
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