Detection of curative effect of antilipemic and antiatherosclerotic drug

An atherosclerotic, antilipidemic technology with applications in molecular biology and medicine

Inactive Publication Date: 2010-12-29
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies have shown that fluvastatin has significant pharmacokinetic differences in individuals of different genotypes, but no significant difference in pharmacodynamics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] Below in conjunction with specific embodiment, further illustrate the present invention. For the experimental methods not indicated in the following examples, the specific conditions are usually in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer.

[0026] The use of embodiment detection kit

[0027] Step 1: Extraction of DNA template

[0028] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0029] Step 2: Real-time quantitative PCR reaction

[0030] Use the fluorescent quantitative PCR kit in the detection kit to perform 3 independent fluorescent quantitative PCR reactions. The total volume of each reaction is 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer , 0.1μl 25mM dNTP mixture, 0.6μl 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0.225 μl each of 20 μM sense primer and antisens...

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PUM

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Abstract

The invention discloses a kit for detecting the curative effect of an individual antilipemic and antiatherosclerotic drug. The kit comprises a specific primer pair, a specific fluorescent probe pair, a fluorescence quantitative PCR conventional assembly and the like, and the specific primer pair is used for detecting a medication target site, namely a CYP3A4 gene, CYP2D6 gene and CYP2C9 gene polymorphism site of the statin drug. The kit can be used for estimating the curative effect of the individual antilipemic and antiatherosclerotic drug by detecting the medication target site, namely CYP3A4 gene, CYP2D6 gene and CYP2C9 gene polymorphism siteCYP3A4 gene, CYP2D6 gene and CYP2C9 gene polymorphism site of the statin drug.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting the curative effect of individual anti-blood lipid and anti-atherosclerotic drugs, by detecting the CYP3A4 gene and CYP2D6 gene targeted by statin drugs and CYP2C9 gene polymorphisms to evaluate the efficacy of individual anti-lipid and anti-atherosclerotic drugs. Background technique [0002] Atherosclerosis is the most common and important of a group of vascular diseases called arteriosclerosis. The common feature of all kinds of arteriosclerosis is that the arterial wall thickens and hardens, loses elasticity and shrinks. The characteristic of atherosclerosis is that the lesions of the affected arteries start from the intima, and there are multiple lesions in succession, including local accumulation of lipids and complex carbohydrates, fibrous tissue hyperplasia and calcium deposition, and gradual re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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