Deubiquitination mutant of mda-7/IL-24
A technology of IL-24 and deubiquitination, applied in the field of genetic engineering, can solve the problems of large difference in composition, small specificity, and inability to inhibit the growth of pancreatic cancer cells
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[0034] Example 1 Preparation of IL-24 Deubiquitination Mutant Polypeptide
[0035] (1) Construction of IL-24 mutants
[0036] The lysines at positions 63, 69, 78, 119, 123, 136, 179, 189, 203, and 206 in the cDNA sequence of IL-24 were mutated into arginines. According to the known IL-24 gene sequence and primer design principles, 10 pairs of primers were designed and synthesized by Invitrogen Biotechnology Co., Ltd.
[0037] Pa1: GCCCTGCCAAGTG AGG GGGGTTGTTGTTCCCCAG
[0038] Pa2: CGGGACGGTTCAC TCC CCCCAACAACAAGGGGTC
[0039] Pb1: GGTTGTTCCCCAG AGA CTGTGGGAAGCCTTC
[0040] Pb2: CCAACAAGGGGTC TCT GACACCCTTCGGAAG
[0041] Pc1: GCCTTCTGGGCTGTG AGA GACACTATGCAAGC
[0042] Pc2: CGGAAGACCCGACAC TCT CTGTGATACGTTCG
[0043] Pd1: CCCTGCTGGAGTTCTACTTG AGA ACTGTTTTTCAAAAACTACC
[0044] Pd2: GGGACGACCTCAAGATGAAC TCT TGACAAAAGTTTTTGATGG
[0045] Pe1: CTTGAAAACTGTTTTC AGA AACTACCACAATAGAAC
[0046] Pe2: GAACTTTTTGACAAAAG TCT TTGATGGTGTTATCTTG
[0047] Pf1: GTCA...
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