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Bud pressing method for grifola frondosa

A technology of Grifola frondosa and mycelium, which is applied in the fields of botany equipment and methods, horticulture, application, etc., can solve the problems such as obstacles to the formation of primordia, and achieve the improvement of the formation rate of primordia, the beautiful shape of fruiting bodies, and the improvement of primordium formation The effect of proportion

Inactive Publication Date: 2011-01-19
SHANGHAI ACAD OF AGRI SCI +1
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a budding-inducing method of Grifola frondosa to solve the problem of barriers in the formation of primordia at the present stage, improve the yield of artificial cultivation of Grifola frondosa, and ensure the success of annual bag planting of Grifola frondosa

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take Grifola frondosa strain No. 1 (preserved by the strain preservation center of the Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, preservation number: 2597) as an example:

[0027] Carry out mycelia inoculation by conventional methods, then place the Grifola frondosa fungus bags at a temperature of 22°C, a relative humidity of 60%, and CO 2 The concentration is 1000-2000ppm, and the culture is carried out in a dark room. On the 10th day, 22d day, and 40th day of culture, the bacterial contamination inspection was carried out. After the hyphae grow through the fungus bag, they need to be post-ripened for 20 days to facilitate the maturation of the mycelia. The physiologically mature bacterial bags were removed from the culture room for incision treatment. Select the thick white part of the mycelium, first wipe the surface of the fungus bag with 75% alcohol for disinfection, and then use a sterilized blade to cut a circular opening with a diam...

Embodiment 2

[0029] Take Grifola frondosa strain No. 2 (preserved by the Culture Collection Center of the Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, preservation number: 2584) as an example:

[0030] Place the bag of Grifola frondosa at a temperature of 22°C, a relative humidity of 60%, and CO 2The concentration is 1000-2000ppm, and the culture is carried out in a dark room. On the 10th day, 22d day, and 40th day of culture, the bacterial contamination inspection was carried out. After the hyphae grow through the fungus bag, it needs to be post-ripened for 25 days to facilitate the maturation of the mycelia. The physiologically mature bacterial bags were removed from the culture room for incision treatment. Select the thick white part of the mycelium, first wipe the surface of the fungus bag with 75% alcohol for disinfection, and then use a sterilized blade to cut a circular opening with a diameter of 1-1.5 cm and a depth of 1 cm to dig out the mycelium. After...

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Abstract

The invention provides a bud pressing method for grifola frondosa. The method comprises the following steps of: inoculation and culture of hyphae, cutting, recovery of hyphae, primary bud pressing, secondary bud pressing and growth period. The method can obviously raise the ratio of forming anlages of the grifola frondosa, and improve the quality of the formed anlages.

Description

Technical field: [0001] The invention relates to the field of edible fungus cultivation, in particular to a budding-inducing method for promoting the formation and differentiation of the primordia for the bag-type annual cultivation of Grifola frondosa. Background technique: [0002] Grifola frondosa has a unique aroma and taste, and has attracted much attention because of its outstanding anti-cancer and health care functions. At present, high-quality health care products have been made from Grifola frondosa (Patent No. 200410083062), and its edible and medicinal value is increasingly recognized by people With acceptance, the future has broad market prospects. At present, in my country, only Qingyuan County in Zhejiang Province and Qianxi County in Hebei Province have established large-scale production bases using natural conditions, and scientific research institutes in various places have carried out small-scale planting experiments. Cultivation experiments show that the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 王瑞娟郭倩张美彦潘辉
Owner SHANGHAI ACAD OF AGRI SCI
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