Gene combination, primer and probe for detecting gastric cancer susceptibility and application

A gene combination, gastric cancer technology, applied in primers, detection of gastric cancer susceptibility gene combination, probe and application field, can solve problems such as bad living habits and changes, and achieve high sensitivity, good repeatability, and high throughput.

Inactive Publication Date: 2011-02-02
NANJING WEIYU GENETIC ENG
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to comprehensively detect and analyze a group of genes and loci related to gastric cancer susceptibility, using specific primers and probes, and combining single nucleotide extension tech

Method used

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Embodiment Construction

[0029]The invention relates to a gene combination for detecting gastric cancer susceptibility, which includes a combination of four genes closely related to gastric cancer: MTHFR gene, CYP2E1 metabolic enzyme gene, XRCC1 and ADPRT repair enzyme gene. Including the following SNP sites: rs1801133 site of MTHFR gene, rs2031920 site of CYP2E1 gene, rs25478 site of XRCC1 gene, rs1136410 site of ADPRT gene.

[0030] The gene combination for detecting gastric cancer susceptibility is used to design specific primer pairs and probes for the site.

[0031] The primers of the present invention are designed for the rs1801133 site of the MTHFR gene, the rs2031920 site of CYP2E1, the rs25478 site of the XRCC1 gene, and the rs1136410 site of the ADPRT gene, and can be used for multiplex PCR amplification, and can simultaneously amplify Gene fragments of the above 4 loci. Designing such primers is readily accomplished by those skilled in the art. Preferably, the primers described in the pre...

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Abstract

The invention discloses a gene combination, a primer and a probe for detecting gastric cancer susceptibility and application. The gene combination comprises a combination of four genes closely related with a gastric cancer, namely an MTHFR gene, a CYP2E1 metabolic enzyme gene, an XRCC1 repairase gene and an ADPRT repairase gene. The gene combination comprises the following SNP sites: rs1801133 site of the MTHFR gene, rs2031920 site of the CYP2E1 gene, rs25478 site of the XRCC1 gene and rs1136410 site of the ADPRT gene. Whether the detected crowds carry 'gastric cancer susceptible genes' are comprehensively detected and analyzed by detecting a group of genes and sites related with the gastric cancer susceptibility, using the specific primer and the probe and combining a mononucleotide extending technique and a micro array chip technique so as to screen the gastric cancer susceptible crowd from the crowds, change unhealthy lifestyles and fulfill the purpose of preventing.

Description

technical field [0001] The present invention relates to a gene combination, primer, probe and use, especially a gene combination, primer, probe and use for detecting gastric cancer susceptibility. Background technique [0002] Gastric cancer is one of the most common malignant tumors of the digestive tract, and is a common and frequently-occurring disease that seriously threatens human health and life. According to statistics, about 900,000 people around the world are newly diagnosed with gastric cancer every year. In my country, gastric cancer ranks first among digestive tract malignancies and third among systemic malignancies. According to the data released by the Health Information Statistics Center of the Ministry of Health in 2001, malignant tumors are the first cause of death among urban residents in my country, with a mortality rate of 135.59 / 100,000 (24.9%). Among them, gastric cancer ranks third in the cause of death from malignant tumors. The occurrence and devel...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 杨亚非
Owner NANJING WEIYU GENETIC ENG
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